Microbiology and it's applications
.::. Invited Lecture
1
26
Novel Molecular Mechanisms for Prevention and Treatment of Chronic Viral Hepatitis(Immune Based Therapy)
Mohamed M. S. Farag
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
ABSTRACT
Chronic hepatitis virus infection is a leading cause of liver fibrosis, cirrhosis and the development of hepatocellular carcinoma. Virus replication can effectively be controlled by potent new direct antiviral agents (DAA). Dendritic cells (DCs) are a promising tool for therapeutic and/or prophylactic vaccination experiments, considering their multiple functions in immune modulation. It is known that in chronic hepatitis B and C virus carriers the function of DCs as well as the function of T-cells and B-cells is impaired and the specific cellular immune responses against virus are down regulated. We used bone-marrow derived dendritic cells from BALB/c mice pulsed with hepatitis B virus subviral particles (HBVsvp) and/or pseudo particles from the hepatitis C virus (HCVpp) to activate dendritic cells in vitro. In the present study, the potential of ex vivo activated DC to induce specific immune responses against HBV and HCV was examined. In our study we used naïve as well as transgenic mice as model for chronic infection to observe immune responses. We were able to demonstrate that it is possible to activate DC of mice in vitro by pulsation with constructed particles and that by immunizing mice with such activated DC specific antiviral directed immune responses are induced on cellular and humoral levels. In addition, increased production of cytokines and T cells proliferation in treated mice compare to untreated control mice, demonstrated that in vitro activated DC can effectively induce immune responses against hepatitis virus in vivo. These data lead to the assumption that DC function infected patients could be restored in vitro, possibly assisting the patients to better control or even clear the virus. The results demonstrate that in vitro activation of DC can overcome the impaired DC and T-cell function in infected patients, as well as vaccination with ex vivo activated DC may be a promising tool for therapeutic or prophylactic approaches against the Hepatitis virus.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
2
26
Essential oil of Foeniculum vulgare Mill as an antifungal and defense inducing agent against root rot disease in Vicia faba L.
Ahmed Mohamed ALy Khalil , Elsayed S. Abd Elrazik , Hayat Zagloul , Mona M. Khaleil
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City for Scientific Research and Technology Applications New Borg EL-Arab, 21934, Alexandria, Egypt.
Chemistry Department, Faculty of Science, Taibah University, Al Sharm, Yanbu El Bahr 46429, Saudi Arabia.
Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt
ABSTRACT
Root rot disease of Fusarium solani is one of the major constraints of beans production in Egypt. Foeniculum vulgare Mill. (F. vulgare), commonly known as Fennel, is a popular medicinal plant with various antioxidant and antimicrobial activities against F. solani KHA10 with accession number MW444555. Here, we tested different concentrations of fennel seed essential oil (FSEO) (0, 0.5, 1.0 and 2.0% v/v) against F. solani KHA10 that could affect its growth in Vicia faba L. Minimum growth (2.2 cm) was observed at 2.0% FSEO. The greenhouse results showed that the highest root protection (21.9% and 33.6% after 3 and 6 weeks of soaking, respectively) was achieved in Vicia faba seeds soaked in 2% FSEO. Disease severity (DS) decreased by 19.3 % and 32.14% after 3 and 6 weeks of sowing FSEO treated faba seeds in the pathogen infested soil. Significant increase in growth and biochemical parameters were recorded in FSEO treated plants compared with P and CK treatments. Plant height increased by 5.54% and 2.8% after 3 weeks and 6 weeks of sowing, respectively. Shoot fresh weights significantly increased by 1.12% and 2.32% after 3 and 6 weeks of sowing as compared to CK. Root fresh weights significantly increased by 0.58 % and 0.84 % after 3 and 6 weeks of sowing as compared to CK. Shoot dry weights significantly increased by 0.17% and 0.18% after 3 and 6 weeks of sowing above control value. Root dry weights significantly increased by 0.1% and 0.14% after 3 and 6 weeks of sowing as compared to CK. Moreover, highest GE levels of defensin and chitinase were (11.84, 11.65, 11.08 and 10.83) achieved in case of FSEO. The chemical composition of fennel oil through gas chromatography/mass spectrometry analysis revealed the presence of ten major compounds in different percentages where most abundant compounds were Cis-vaccenic acid (31.23%) followed by 9, 12 Octadecadienoic acid (29%). Therefore, it can be concluded that FSEO can be used as a safe biocontrol agent in control of Fusarium root rot disease of broad bean.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
3
26
Synthesis of composite based on cellulose and amino acid loaded graphene oxide: antimicrobial and antiviral activity
Amr H. Hashem , Mohamed Hasanin , Samir Kamel , Sawsan Dacrory
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt.
Cellulose and Paper Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt
Cellulose and Paper Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt
Cellulose and Paper Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt
ABSTRACT
In this work, biocompatible, antimicrobial, and antiviral composites were prepared through two steps. In the first step, periodate oxidation of cellulose was performed to get dialdehyde cellulose (DAC). The second step included the reaction of DAC with sulfur-containing amino acids such as Cysteine (Cys) and Methionine in the presence of graphene oxide (GO). The prepared composites were characterized via FT-IR, SEM, TEM, and TGA. Antimicrobial and antiviral activity for all designed composites besides DAC were carried out. Both DAC/GO/Cys and DAC/GO/Meth had a promising antimicrobial activity against gram-negative (E. coli and P. aeruginosa), gram-positive (B. subtilis and S. aureus), and unicellular fungi (C. Albicans and C. neoformans), while the DAC/GO/Cys/Meth composite was the lowest. Moreover, all designed composites had strong antiviral activity against HSV-1 at minimum nontoxic concentration. Additionally, Computational procedures and Molecular docking showed the reactivity and stability of the molecules that have biological activity against Gram-positive, Gram-negative, and Herpes simplex virus. As well as DAC incorporation with amino acid enhanced their reactivity and their interaction.
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BIO
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
4
26
Synthesis of composite based on cellulose and amino acid loaded graphene oxide: antimicrobial and antiviral activity
Amr H. Hashem , Mohamed Hasanin , Samir Kamel , Sawsan Dacrory
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt.
Cellulose and Paper Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt
Cellulose and Paper Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt
Cellulose and Paper Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, 12622, Egypt
ABSTRACT
In this work, biocompatible, antimicrobial, and antiviral composites were prepared through two steps. In the first step, periodate oxidation of cellulose was performed to get dialdehyde cellulose (DAC). The second step included the reaction of DAC with sulfur-containing amino acids such as Cysteine (Cys) and Methionine in the presence of graphene oxide (GO). The prepared composites were characterized via FT-IR, SEM, TEM, and TGA. Antimicrobial and antiviral activity for all designed composites besides DAC were carried out. Both DAC/GO/Cys and DAC/GO/Meth had a promising antimicrobial activity against gram-negative (E. coli and P. aeruginosa), gram-positive (B. subtilis and S. aureus), and unicellular fungi (C. Albicans and C. neoformans), while the DAC/GO/Cys/Meth composite was the lowest. Moreover, all designed composites had strong antiviral activity against HSV-1 at minimum nontoxic concentration. Additionally, Computational procedures and Molecular docking showed the reactivity and stability of the molecules that have biological activity against Gram-positive, Gram-negative, and Herpes simplex virus. As well as DAC incorporation with amino acid enhanced their reactivity and their interaction.
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GEO
BIO
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
5
26
The use of indigenous ethnomedicine in controlling some growth criteria of the opportunistic fungus Aspergillus fumigatus
Eman F. Sharaf , Hanan S. Al-Zaidi
Department of Biology, Faculty of Science, Taibah University, AL-Madinah AL- Munawarah, Kingdom of Saudi Arabia.
Department of Biology, Faculty of Science, Taibah University, AL-Madinah AL- Munawarah, Kingdom of Saudi Arabia.
ABSTRACT
Aspergillus fumigatus is a respiratory tract opportunistic fungus causes pulmonary diseases to humans. Cinnamon, a medicinal plant used in traditional medicine, is currently chosen to investigate its antifungal activity against this fungus. The park of the cinnamon tree, purchased from a market in AL-Madinah AL- Munawarah, was milled and extracted by hot water and ethanol. Both extracts showed an inhibitory activity against five isolates of Aspergillus fumigatus, with ethanol extract being more effective especially against Aspergillus fumigatusI2. Accordingly, the effect of different concentrations (20- 200mg/ml) of cinnamon ethanol extract was tested on some growth criteria of the fungus. The extract showed a concentration-dependent reduction in linear growth of A. fumigatus I2, and its dry weight. Ethanol extract strongly reduced the polysaccharide and protein contents of the fungal mycelia which reflects strong antifungal activities. GC-MS phytochemical screening of both ethanol and aqueous cinnamon extracts revealed the presence of a high quantity of basic component cinnamaldehyde, followed by benzoic acid, benzaldehyde and volatile oils which were highly concentrated in the ethanol than aqueous extract. Cinnamon and eugenol were present in the ethanol extract only but anisole was present in the aqueous one. From this study, it could be suggested the use of cinnamon ethanol extract to treat pulmonary diseases resulting from the opportunistic A. fumigatus instead of the chemical drugs that have different side effects and to the appearance of microbial resistance against their repeated use.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
6
26
Molecular and Biochemical Studies on Hepatitis C Patients Relapsed After Sofosbuvir Treatment
Ayman A. Farrag , Amal R. Mansour , Mostafa H. Diab , Mohamed A. El Badry
Department of Botany and Microbiology, Faculty of Science, Al-azhar University, 11884 Nasr City, Cairo, Egypt
MD, PhD, Professor Department of Clinical Pathology, Alexandria Faculty of Medicine, Alexandria University, Egypt.
Msc Student
Department of Botany and Microbiology, Faculty of Science, Al-azhar University, 11884 Nasr City, Cairo, Egypt
ABSTRACT
Introduction: Hepatitis C viral infection is one of the most common diseases in Egypt in the 20th century that leaded to liver diseases such as liver cirrhosis or hepatocellular carcinoma (HCC).
Sofosbuvir is one of the most successful direct-acting antivirals (DAA) that play an important role in treatment of HCV.
Methods: Seventy egyptain patients with HCV infection and decompensated cirrhosis were administered sofosbuvir combined with other drugs such as Ribavirin as 1st therapy or Simeprevir as 2nd therapy between Oct 2015 to Oct 2017.
Results: All of our cohort treatment experienced were between 1st and 2nd therapy including monitoring for their liver functions after 1st treatment, during relapse period and after receiving the 2nd therapy. Patients that were treated with combination of Sofosbuvir and Ribavirin at the first therapy achieved SVR after 24 weeks, a decrease in liver functions (ALT & AST) 94% achieving normal values, while 6% had mid elevation in ALT; 84% had normal values of AST and 16% had abnormal results with a negative PCR results. After follow up (4 w to 48 w) 36 % had normal ALT, 64% had abnormal results; 11.2 % had normal AST, 89% were abnormal. Patients were relapsed in 48weeks, and their viral load ranged from 7.0 X 101 to 9.90 X 106IU/m. After receiving the 2nd therapy, patients achieved SVR in a period ranged from 4 weeks to 12 weeks with no apparent changes in ALT, AST values, while the viral load was negative in all patients.
Conclusion: Combination therapy (Sofosbuvir and Simeprevir ) for 12 weeks in patients with geneotype 4 infection is an effective regimen with an overall SVR rate of 100%.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
7
26
Detection of Antimicrobial Resistant Genes in Diverse Bacterial Groups as Opportunistic Nosocomial Pathogens
Ayman A.Farrag , Waleed B. Suleiman , Mohammad Shawky
Botany & Microbiology Department, Faculty of Science, Al-Azhar University, Cairo-Egypt.
Botany & Microbiology Department, Faculty of Science, Al-Azhar University, Cairo-Egypt.
Botany & Microbiology Department, Faculty of Science, Al-Azhar University, Cairo-Egypt.
ABSTRACT
Bacterial infections represent a very serious problem threaten the human health, antibiotics were designed to attack the causative agents of infectious diseases, but some bacterial pathogens became virulent and resistant to antibiotics with different mechanisms, one of those mechanisms belong to genetic resistance. This study attempts to investigate some clinical bacterial isolates as well as some other bacterial groups provided by the culture collection unit of the center of fermentation biotechnology, Al-Azhar university, Cairo-Egypt, for example; Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Salmonella typhi, Klebsiella pneumonia and other clinical samples recovered from patients, the total number of these isolates are 25 different isolates. Response of these bacterial isolates to different 12 antibiotic discs were reported, and the report showed that the provided bacterial strains are slightly susceptible except P. aeruginosa and K. pneumonia which could be considered as multidrug resistant strains, but in case of the clinical samples; isolate number 12 and 17 are completely resistant to all tested antibiotics, isolate number 2, 7 and 12 are susceptible only for two antibiotics as like as the rest of the clinical isolate have the property of multidrug resistance. Therefore, detection of resistance gene became crucial and critical to recognize the mechanism of resistance, five pairs of primers were included to investigate five responsible genes belong to beta lactamases, efflux pump and methicillin resistance. Conclusively, all of those genes were detected in all tested bacterial pathogens as a clear evidence which approved the relation between phenotype and genotype because the resistance mostly occurred and appeared phenotypically as a result to presence of some genes responsible for secretion of some resistance enzymes thus PCR technique is very accurate tool to check the resistance whether detected at present or expected in future.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
8
26
Evaluating the Performance of routine phenotypic methods for accurate and rapid detection of carbapenem-resistant Enterobacteriaceae in clinical samples
Ayman A. Farag , Mohamed Ali Abdel-Rahman , Amani El-Kholy , Arwa R. Elmanakhly , Elsayed Gamal Nana
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University,
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University,
The Departments of Clinical Pathology, Faculty of Medicine, Cairo University,
Lecturer of microbiology and immunology, faculty of pharmacy, Russian university in Cairo
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University,
ABSTRACT
Background and Aim: The emergence and spread of carbapenem resistant Enterobacteriaceae (CRE) have become an increasing concern for healthcare services worldwide. Carbapenem-resistance in Enterobacteriaceae is mostly due to production of carbapenemases of various classes, which can hydrolyze carbapenems and other beta-lactam antibiotics. Infections caused by these bacteria have been associated with significant morbidity, mortality, prolonged hospitalization and treatment options. The rapid and accurate detection of CRE is important for infection control to limit the spread of these bacteria as well as clinicians to ensure adequate and appropriate use of antimicrobial agents. Thus, the aim of this study was to evaluate the performance of the phenotypic and genotypic methods used for detection of CRE strains isolated from patient hospitalized in intensive care (ICU) unite of tertiary hospital in Egypt over a period from January 2017 to April 2019.
Methods: The study included 65 CER isolates. The isolates were initially identified as CRE on the basis of their reduced susceptibility to MEM, IPM or ETP. Antibiotic sensitivity was performed according to (CLSI, 2017) guidelines. All the isolates were identified by standard microbiological procedures as traditional biochemical reactions for entrobacteriaceae species then re-identified using API20E (Bio-Mérieux, Marcy l'Etoile, France), and Matrix-Assisted Laser Desorotion/Ionisation Time-Of-Flight Mass Spectrometry (MALDI-TOF/MS). CRE isolates were screened for the presence of carbapenemase genes by routine phenotypic methods including Modified Hodge Test (MHT), EDTA-Combined Disc Test (EDTA-CDT) and modified Carbapenem Inactivation Method (mCIM). Detection of the five common and predominant carbapenemases genes (blaKPC, blaNDM, blaOXA-48, blaVIM, and blaIMP) was performed using multiplex PCR.
Conclusion: Carbapenem resistance in Egypt is increasing, and OXA-48 is the most common source of carbapenem resistance in Enterobacteriaceae in our country. Elderly population and ICU admission were important risk factors for CRE acquisition. Accurate detection of carbapenemase producing genes by molecular methods overcomes the problem related to CRE. Though there is no signal method that is ideal for all situations, the mCIM method is simple, less subjective, cost effective, reproducible and most sensitive method for detection of CRE.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
9
26
Microbiological quality and regulatory compliance of drinking water in Egypt
Ayman A. Farrag , Elhassan A. Abdel-Sadek
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
bReference Lab. For drinking water, Holding Company For water &wastewater, Cairo, Egypt.
ABSTRACT
In recent decades, water contamination has become a rising threat to humanity and natural ecosystem, which demands the need to investigate and comprehend the space and time variabilities of pollutants. Assuring sufficient quality of drinking water is of great importance for public wellbeing and prosperity. Egypt has developed a national regulation with the aim of providing drinking water of sufficient quality and to minimize the risk of contamination of the water supply in the first place.
Microbiological analysis survey was conducted for drinking water samples from 2016–2019 from 24 Egyptian governates. All analysis was conducted according to latest version of Standard Methods for Examination of Water and Wastewater (2017) using membrane filtration technique. A total of 2847 water samples (surface water treatment plant outlet n = 1108; ground water treatment plant outlet n = 374; and distribution lines n = 1392) were analyzed.
Overall, 3.09 % and 1.72 % of water samples were positive for total coliform (TC) and fecal coliforms (FC), respectively. Furthermore, 39.26%, 8.07%, and 31.4% of surface water treatment plant outlet, ground water treatment plant outlet, distribution lines samples were positive for protozoa, respectively. As a general, 68.6% of samples were comply with Egyptian regulations.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
10
26
Optimal conditions for decolorization of azo dyes by fungal isolates
Salem S. Salem , Amr Fouda , Mohamed Talat , Mamdouh S. Gl-Gamal , Asem A. Mohamed
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
National Research Center, El-Behouth St. (Former El-Tahrir Str.), Dokki, P.O. 12622, Giza, Egypt.
ABSTRACT
Background: Different technologies may be used for decolorization of wastewater containing dyes. Among them, biological processes are the most promising because they seem to be environmentally safe. In this study, fungal isolates A2 and G2-1 were isolated from contaminated soil collected from textile industry companies and showed high potentiality for decolorization and degradation of two different azo dyes.
Results: The most potent fungal isolates were identified as Aspergillus sp. (A2) and Fusarium sp. (G2-1) using morphological and cultural characteristic. Decolorization process under different optimized condition of azo dye concentration, pH, incubation periods, inoculum size and different carbon and nitrogen sources were investigated. The maximum decolorization efficiency against 100 ppm dye concentration of reactive yellow (4GL) and reactive red (4BL) dyes of 87.00±0.40% and 81.73±0.80%, respectively, were recorded for Aspergillus sp at pH 8.0, in presence of 2 % glucose and 0.5% yeast extract as carbon and nitrogen sources respectively at room temperature after 7 days on shaking conditions. Similarly, the maximum decolorization efficiency against 100 ppm dye concentration of reactive yellow (4GL) and reactive red (4BL) dyes of 68.42±0.01% and 63.31±0.0%, respectively, were recorded for Fusarium sp at pH 6.0, in presence of 2 % glucose and 0.5% yeast extract as carbon and nitrogen sources respectively at room temperature after 7 days on shaking conditions. Ultra violet-visible (UV-Vis) spectrum analysis of the treated yellow and red dyes showed complete disappearance of the peaks at ~ 425 nm and at ~ 520 nm, indicates the degradation of dyes due to fungal action.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
11
26
Identification and Distribution of Pathogenic Bacteria in Clinical Specimens Within Egypt and Surrounding Countries
Khaled S. M. Azab , Mohamed Ali Abdel-Rahman , Mohamed M. S. Farag , Hussein H. El-Sheikh
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
ABSTRACT
Background: Pathogenic bacteria contribute to many globally important diseases, such as pneumonia, urinary tract infection, foodborne illnesses, and others.
Aim: The present study aims to study and survey different isolates of pathogenic bacteria from different countries to assess their prevalence and their relation to diseases.
Methods: Two hundred and ninety-two clinical samples were collected randomly from private laboratories and Hospitals of three countries (Egypt, Saudi Arabia, and Sudan). All samples were collected from patients (male and female) for different age groups during the period 2015-2016. Samples included the present study were urine (168), vaginal swab (43), ear swab (22), blood (19), abscess (17), endotracheal tube (8), sputum (8), throat swab (3), nasal swab (3), and urethral swab (1). All samples were inoculated on different suitable culture media. After growth, isolated bacteria were identified by physiological and biochemical properties.
Results: Mainly 5 bacterial genera were detected amongst all clinical samples. The isolates were identified to be E. coli (103), Klebseilla spp. (47), Proteus spp. (14), Pseudomonas spp. (83), and Staphylococcus spp. (45). Regarding patient gender, the presented study showed that females had a tendency to get infected more than males, where 110 (37.67 %) of patients were males and 182 (62.33 %) were females. The most common microbes in urinary tract infection were Escherichia coli. The most common microbes in acute otitis externa were Pseudomonas aeruginosa and Staphylococcus aureus. All isolates from abscesses were Pseudomonas aeruginosa and Staphylococcus aureus. The results showed that Pseudomonas aeruginosa was the most common isolated samples and only Proteus mirabilis were isolated urine samples.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
12
26
Development of a Cost-effective Lactic Acid production system Through Organic Waste Valorization
Mahmoud Ali Gaber , Saad El-Din Hassan , Mohamed Ali Abdel-Rahman , Mohamed M. Roushdy , Mohamed Salah Azab
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, P.N.:11884, Nasr City, Cairo, Egypt.
ABSTRACT
Lactic acid (LA) has a broad range of applications in foods, cosmetics, pharmaceutical, and medical industries. This study aimed to develop a cost-effective system for LA production from different waste materials under harsh conditions. Several lactic acid-producing bacteria were isolated from different natural sources. The isolate FW26 was considered as the most potent LA producer by direct utilization of banana peels at pH 9.0 and 50 ○C. This isolate was identified as Enterococcus faecium FW26 strain using 16S rRNA sequencing. The optimal solid-liquid ratio was 1:10 (w/v) at which 15.9 g/L was produced. A positive synergistic effect was achieved with the co-utilization of banana peels with food waste materials at 1:1.5 (w/w) which attaining 19.3 g/L of LA. Interestingly, the co-utilization without medium supplementation was achieved with comparable values of LA fermentation parameters. Surprisingly, controlling the pH of the fermentation medium resulted in 81% improvements and achieved LA production at 33.3 ± 2.6 g/L without pretreatments or nutrient supplementation. This study introduces a cost-effective system by co-waste valorization for LA production under high-stress conditions that would greatly overcome the contamination risk.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
13
26
Plant Growth Promoting Potential of Bacterial Endophytes Associated With
Pulicaria Incisa (Lam.) DC
Ahmed Eid Mohamed Mohamed Eid
Al-Azhar University, Cairo, Egypt
ABSTRACT
Endophytes are the microbes that residing in plant tissues without causing harmful effects to the host plant. Almost all plants in the world have been found to be associated with endophytes. Endophytic bacteria are of agrobiological interests because they create host–endophyte relationship having exciting prospects for newer biotechnological applications. Therefore, the aims of this study were to identify bacterial endophytes of Pulicaria incisa and to characterize plant growth-promoting (PGP) properties of these endophytes. Two representative bacterial endophytes were isolated and identified as Bacillus cereus and Bacillus subtilis. The isolated endophytes differentially produced indole acetic acid (IAA) and ammonia, in addition to their phosphate solubilizing capacity and extracellular enzymatic activities. In order to investigate the effect of endophytes on root length of maize plants, maize seeds were individually inoculated with the bacterial endophytes and the gnotobiotic root elongation assay was performed. The results proved the potency of the isolated bacterial endophytes for enhancing root length and it could be used as inoculants to establish a sustainable crop production system.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
14
26
The Potential of Newly Isolated Thermotolerant Yeast, Candida parapsilosis NH-3,
for Citric Acid Production from agro-industrial waste
Abdelrahman Khattab , Mohamed Ali Abdel-Rahman , Said El-Sayed Desouky , Mamdouh Salem El-Gamal
Botany and Micbiology Department, Faculty of Science (Boys), Al-Azhar University
Botany and Micbiology Department, Faculty of Science (Boys), Al-Azhar University
Botany and Micbiology Department, Faculty of Science (Boys), Al-Azhar University
Botany and Micbiology Department, Faculty of Science (Boys), Al-Azhar University
ABSTRACT
Sugar cane molasses was employed in this work as raw material for the production of citric acid (CA) by submerged fermentation (SmF). High potential, thermotolerant, CA-producing yeasts were successfully isolated in this study. The isolate NH-3 was obtained from Lareng Soil at El-Behirah District, Egypt and selected for identification and further work. Based on macroscopic, microscopic, and molecular characteristics, this isolate is identified as Candida parapsilosis NH-3. The effects of the main operating variables include the temperature (20–50 oC), the pH values (2–6), the molasses concentration (10%–50%), different molasses treatment methods, nitrogen and phosphorus limitation in the production media were investigated. Moreover, evaluating the effect of different alcohol types and statistical optimization using Plackett-Burman design were studied. In this work the optimal conditions for CA production revealed to be at temperature 40±1°C, pH 5.0, molasses concentration 30%, pre-treatment of molasses using EDTA, nitrogen and phosphorus limitation excluding dipotassium hydrogen phosphate, and alcohol addition of 1% methanol. Under these conditions, the SmF ensured an effective specific production of CA 4.30 g/L after 24 h. These results, which demonstrate the viability of the CA production by SmF from sugar cane molasses using for the first time Candida parapsilosis NH-3, could be of interest to possible future industrial applications.
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CSC
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
15
26
The influence of discharge effluents on River Nile water quality at Aswan Governorate, Egypt
Sayeda M. Ali , Ahmed D. El-Gamal , Magdy M. Afifi , Amr H. Fouda , Hesham A. Abo El-Magd
Microbiology Department, National Institute of Oceanography and Fisheries, Aswan Research Station, Egypt.
Botany and Microbiology Dept., Faculty of Science (boys), Al-Azhar University, Cairo, Nasr City, Egypt.
Botany and Microbiology Dept., Faculty of Science (boys), Al-Azhar University, Assiut, Egypt
Botany and Microbiology Dept., Faculty of Science (boys), Al-Azhar University, Cairo, Nasr City, Egypt.
Botany and Microbiology Dept., Faculty of Science (boys), Al-Azhar University, Assiut, Egypt
ABSTRACT
Assessment of water quality remains the main target in Egypt and all the world, to preserve people's health and obtain good quality industrial and agricultural products. In this study we are evaluate the water quality of 15 sites along Aswan governorate, which represented five sectors with three samples from each sector (east, west and middle), four sectors represented main visible discharge drains (El-Sail, Kom Ombo, Edfu ferroalloys and Edfu sugar cane factory) and one sector represented non visible discharge effluents (Gabal Taqouq).
The microbiological quality of the water samples was poor at the sites under the influence of the pollution sources. The site of Kom Ombo drain showed the highest values for total viable bacterial counts (TBC) at 22 ºC (1800 x103 cfu/ml), thermophiles (259 cfu/ml) and spore-forms (15.8 x103 cfu/ml), while higher TBC at 37 oC (137 x103 cfu/ml) was recorded at El-Sail drain. Significantly (P<0.05) the highest values for indicator bacteria (Total Coliforms, TC, Faecal Coliforms, FC and Faecal Streptococci, FS) were recorded at El-Sail drain samples. Seasonal variations showed that the highest counts of indicator bacteria (TC, FC and FS) were detected in the warmer seasons (spring and summer). Salmonella sp. and Shigella sp. were detected at about 22 % and 30 % of samples respectively, while E. coli was detected at all sites, with the highest values at El-Sail drain samples.
The results of chemical analyses for water samples showed that El-Sail drain samples recorded the highest values for EC, TDS, BOD, NH3, NO2 and PO4 while Kom Ombo drain samples recorded the highest values for water temperature and NO3. The water quality index (WQI) of the studied areas indicated that, all sites under investigation were (Good) in their WQI ranks, except the sites affected by pollution sources which ranged from (Fair) to (Marginal) in their WQI categories.
In conclusion, the results demonstrate that the River Nile water at Aswan governorate is heavily polluted at certain sites (El-Sail drain and Kom Ombo drain) and this consequently has harmful effect to the aquatic environment. So, treatment and safe disposal of different industrial and domestic wastes is recommended.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
16
26
Prevalence of extended spectrum β-lactamase (ESBL) producing Enterobacteriaceae in nosocomial versus community settings.
Amr Fouda , Gamal M. El-Sherbiny , Mona M. A. Halim , Mohamad Saleh Sadek
Botany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo-11884, Egypt
Botany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo-11884, Egypt
Clinical pathology Department, Faculty of medicine, Cairo University, Cairo, Egypt
Botany and Microbiology Department, Faculty of Science, AL-Azhar University, Nasr City, Cairo-11884, Egypt
ABSTRACT
The occurrence of extended-spectrum β-lactamase (ESBL) producing isolates represents a clinical challenge. In this study, amongst 230 bacterial species isolated from Egyptian pediatric hospital, ESBL producing strains were represented 40% (40 /100) of outpatients, 72.3% (47/65) of on-admission patients and 84.6% (55/65) of inpatient samples collected after 48 hours of admission. Data showed that, 47.69% (31/65) of inpatient cases have acquired ESBL activity due to hospitalization. Physiological and biochemical identification of ESBL producing strains revealed that, Klebsiella pneumonia was the most common ESBL producer. Antibiotic sensitivity of ESBL strains showed that, 20% of outpatient isolates, 51.1% of on-admission and 76.4% of inpatient samples which taken after 48 hours of admission were resistant to aminoglycosides. On the other hand, 15%, 57.5% and 81.9% of outpatient, on-admission inpatient and inpatient samples collected after 48 hours of hospitalized were resistant towards Fluoroquinolones, respectively. The resistance assay of ESBL producing strains against polypeptides were 2.5 %, 6.4% and 9.1% of outpatient, on-admission inpatient and inpatient samples collected after 48 hours inpatient isolates, while were 7.5 %, 38.3% and 67.3% respectively for Carbapenems.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
17
26
New report in microbiological risk assessment in food-contact packing materials
Ayman A . Farrag , Mohammed Abu-Elghait , Ahmed Y. Bedier
Department of Botany and Microbiology, Faculty of Science, Al-azhar University, 11884 Nasr City, Cairo, Egypt
Department of Botany and Microbiology, Faculty of Science, Al-azhar University, 11884 Nasr City, Cairo, Egypt
Department of Botany and Microbiology, Faculty of Science, Al-azhar University, 11884 Nasr City, Cairo, Egypt
Department of Botany and Microbiology, Faculty of Science, Al-azhar University, 11884 Nasr City, Cairo, Egypt
ABSTRACT
Packaging materials are the sum of the elements that are part of the food product and are sold with it in order to presser its contents. In this study, samples were taken from different places such as the workers, production line, raw materials, and finished products in order to identify potential microbes that may lead to microbial contamination of the finished product and then may be migrated to the food itself. It was found that 16.4% of the samples showed bacterial contamination, 8.6% isolated from workers, 2.1% found in the production line, and 5.7% are from the finished products primarily identification by mean of Gram's stain, coagulase, and DNase tests in addition to microscopic examination revealed; 25% of total isolates were coagulase-positive staphylococci (CoPS) while 75% were found to be Coagulase-negative staphylococci (CoNS) strains. The antibiotic susceptibility pattern for these isolates has also been done by antibiotics related to diverse groups, such as Cefoxitin, Amikacin, Erythromycin, Gentamycin, Vancomycin, and others. It was found that 2.2% of the total isolates related to finished products samples (0.9 % are CoPS and 1.3% CoNS) are resistant to diverse antibiotics while 2.9% of bacterial isolates from the production line samples (they were all CoNS) are resistant to different groups antibiotics and 9% of the total workers isolates (1.5% CoPS and 7.5% CoNS) are resistant to several groups of antibiotics. recommendations were requested, including the implementation of GMP, HACCP, and hygiene rules.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
18
26
Pioneer Bacterial route to Zinc oxide Nanoparticles: Extracellular Biosynthesis, Characterization and Biotechnological Applications.
Abdullah M. Abdo
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
ABSTRACT
Bacterial extracellular zinc oxide nanoparticles biosynthesis has attracted interest in nanotechnology as an alternative approach to chemical and physical methods due to their low-cost availability, biocompatibility and their ecofriendly properties. In this research study, three bacterial isolates (AMA-1, AMA-2 & AMA-3) were isolated from soil to screen their ability to extracellular biosynthesize of zinc oxide nanoparticles based on the optical surveillance of color alteration from colorless into turbid white within 24hrs. and UV-visible spectrophotometry at range of (200-800 nm). The most potent isolate that showed the highest plasmon resonance by UV- spectroscopy at UV- spectrum of 380 nm of biosynthesized ZnoNps was subjected to identification by Vitek 2 system analysis and identified as: Pseudomonas aeruginosa with 99% phenomenal Probability. Also, the biosynthesized ZnoNps was characterized by TEM, XRD and FTIR. Moreover, as a biotechnological application the biosynthesized ZnoNps was used to repress the growth of some pathogenic microorganisms significantly and demonstrate prospering larvicidal effectiveness against insect larvae. The overall results indicate promising frontier for the uses of biosynthesized ZnoNps in controlling of infectious diseases and biocontrol of insect larvae.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
19
26
Preliminary Characterization and Identification of Gram Positive
Hemolysis Bacteria
Mohammed G. Barghoth , Said E. Desouky , Ahmed T. Almwafy
Department of Botany and Microbiology, Faculty of Science (Boys), Al-azhar University, 11884 Nasr City, Cairo, Egypt.
Department of Botany and Microbiology, Faculty of Science (Boys), Al-azhar University, 11884 Nasr City, Cairo, Egypt.
Department of Botany and Microbiology, Faculty of Science (Boys), Al-azhar University
ABSTRACT
One of the most important pathogens that threaten human health all over the world is Staphylococcus spp. characterization and identification
of such pathogen considers a useful tool to control of some serious
problems resulting by these bacteria. Therefore one hundred and twenty
samples including blood, urine, abscess, semen, pus, sputum, ears,
vaginal and spit swabs were collected from patients of Tanta University
Hospital and outpatient clinics. A total of 126 Gram positive bacterial
isolates were obtained from these clinical specimens. Out of these isolates
107 bacterial strains were identified as Staphylococcus spp. and 15 strains
identified as Streptococcus spp in addition to 4 strains were identified as
Enterococcus spp. this preliminary identification was conducted on the
basis of colony characteristics such as gram staining, pigment production,
hemolysis, catalase activity, coagulase test and fermentation of manitol
sugar. Out of these strains, 30.95% have the potency to make alpha
hemolysis while 30.95% have the ability to cause beta hemolysis and
38.09% posse the capacity to make gamma hemolysis on blood agar
medium. Beta hemolysis Staphylococcus spp. were selected for study of
some virulence factors on basis of coagulase production in which 17.24%
of Staphylococcus spp. were coagulase positive and 82.76% were
coagulase negative Staphylococcus spp. Studying the susceptibility
pattern of these strains to some commercial antibiotics was carried out.
Further future studies are recommended to investigate the effect of some
natural compounds on gene regulation that responsible for hemolysis
process.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
20
26
Anew Trials For Bio-formation Of Antimicrobial Agent Controlling Multi Drug Resistant Microorganism
Prof.Dr Ayman A . Farag , Prof.Dr Mahmoud Mohamed Elaasser , Dr. Mohamed Ali Elbadry , Ahmed Sadoun Mohamed
Professor of Microbiology and Director of Al-Azhar Center for Fermentation Biotechnology and Applied Microbiology, Faculty of Science, Al-Azhar University, Cairo, Egypt
Assistant professor of Microbiology - The Regional Center for mycology and mycology and biotechnology, Al-Azhar University, Cairo, Egypt
lecture of Microbiology - Faculty of science Al-Azhar University, Cairo, Egypt
Master's student - Faculty of science Al-Azhar University, Cairo, Egypt
ABSTRACT
In the present investigation, a trial was done to find a new antimicrobial agent producing microbe from soil microbiota of local habitats to control the problem of multiple drug resistance. Seventy six isolates were isolated from different localities in Egypt and assayed against pathogenic tested microorganisms using agar well diffusion method. Among of these isolates tested, the isolate (S1SHA1) showed the highest antimicrobial activity against pathogenic test organisms. This isolate was identified as Streptomyces griseoplanus by morphological, physiological, biochemical characters and 16s rRNA gene sequence. Physical and nutritional factors affecting activity of antimicrobial agent were studied. The results showed that, optimum activity of antimicrobial agent achieved with pH 7, incubation temperature 28C, incubation time 7 days, agitation 150 rpm, carbon and nitrogen source starch 1.5% and potassium nitrate 0.4%, as well as phosphorus and NaCl at concentration of 1 %. Antimicrobial agent from batch culture was subjected to extraction and purification processes using ethyle acetate and preparative TLC, respectively. The pure compound resulted from purification process that showed the highest activity against pathogenic tested organisms was analyzed using NMR, FTIR and mass spectroscopy technique. Cytotoxic studies showed that it is not toxic on normal cells.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
21
26
Antimicrobial potency of Humulus lupulus against some food spoilage pathogens
Basma Hamdy , Marwa Abdel Aziz , Sabah Sobhey Mohammed , Mohammed Yosri
The Regional Center for Mycology and Biotechnology-Al-azhar University
The Regional Center for Mycology and Biotechnology-Al-Azhar University
The Regional Center for Mycology and Biotechnology-Al-azhar University
The Regional center for Mycology and Biotechnology Al-Azhar University
ABSTRACT
Inhibition of food spoilage pathogens is usually accomplished by use of chemical preservatives which have harmful impacts on human health. So, the necessity to search for active, safe, natural alternative agents is increased. Plant extracts have been used to preserve foodstuff. Antimicrobial activity of Humulus lupulus methanolic extract was investigated against Gram-positive bacterium (Staphylococcus aureus), Gram-negative bacterium (Salmonella typhi ) and the fungus Aspergillus niger. Methanolic extract of Humulus lupulus was potentially effective against the tested microorganisms and Salmonella typhi was the highest susceptible strain . Further separation of the crude methanolic extracts using column chromatography and TLC plates to obtain the purified antibacterial agent was performed. Using spectroscopic analysis techniques as IR, H1NMR and mass spectroscopy, the active antibacterial agent was characterized as (2E,4E,6E,8E) -9-4,9- dihydro-6H-furo [2,3,-9] chromen-8-yl)-5, 8- dimethylnona-2, 4, 6, 8- totraen-1-al. Also, the ultrastructure changes of tested microorganisms due to treatment by the most active compound using Transmission Electron Microscope (TEM) were studied. The results suggest that the isolated compound of Humulus lupulus methanolic extract supports the traditional medicines (herbal extracts) to cure many diseases and could be a potential antimicrobial agent for further exploitation in food preservation agents.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
22
26
Isolation and identification of Cellulose Producing Bacterial Strain isolated from waste fruit juice
Mamdouh S. El-Gamal , Saad El Din Hassan , Said E. Desouky , Tharwat I. Shaheen , Ahmed Alemam
Department of Botany and microbiology, Faculty of science, Al-Azhar University, Cairo, Egypt.
Department of Botany and microbiology, Faculty of science, Al-Azhar University, Cairo, Egypt.
Department of Botany and microbiology, Faculty of science, Al-Azhar University, Cairo, Egypt.
Textile Industries Research Division, National Research Centre, Dokki, Cairo, Egypt.
Department of Botany and microbiology, Faculty of science, Al-Azhar University, Cairo, Egypt.
ABSTRACT
A bacterial cellulose (BC) producing strain isolated from fermented fruit juice. Twenty BC producing bacteria were isolated from each the isolation sources ( fermented juices). The most potent strain was identified to be komagataeibacter sp. based on several morphological characteristics, biochemical tests. The komagataeibacter sp. Was produce BC within pH 4–9 and exhibit maximum BC production (1.8 g/L) at pH 6 in under static conditions for 7 days. The structure of BC produced from the tested strains was assayed by scanning electron microscope, It was revealed the diameter of thin ribbons ranged from 34.34 nm to 39.16 nm and exhibits higher water content ability (99.5%). In comparison with the specimen from model BC producer, Gluconacetobacter xylinus 10245. Based on these analyses, the isolated komagataeibacter sp. can efficiently produce BC, which can be applied for industrial manufacturing with potential features.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
23
26
Biodegradation of phenanthrene and pyrene by Pseudomonas putida H 18 isolated from contaminated soil
Ahmed Bahgat Adly , Nagwa M. Sidkey , Saad El Din Hassan
Department of Botany and microbiology, Faculty of science, Al-Azhar University, Cairo, Egypt.
Department of Botany and microbiology, Faculty of science(Girls), Al-Azhar University, Cairo, Egypt.
Department of Botany and microbiology, Faculty of science(Boys), Al-Azhar University, Cairo, Egypt.
ABSTRACT
Polycyclic aromatic hydrocarbons (PAHs) are the major sources of pollution that cause dangerous effects on human and other organisms. Biodegradation of PAHs in the contaminated area is an engaging remediation technique and its accommodation depends on the optimal condition for the PAH-degrading isolates. In the current study, four bacterial strains were isolated from polluted area with petrochemical compounds with the ability for biodegradation of phenanthrene and pyrene. Only one strain has high biodegradation ratio of phenanthrene and pyrene. The optimization process for biodegradation of phenanthrene and pyrene was executed and qualified under different conditions of shaking, static, pH, temperature, inoculum sizes, salt concentration, carbon and nitrogen sources. Phylogenetic tree based on 16S rDNA genetic analysis sequence indicates that this bacterial isolate was belonged to genus pseudomonas and identified as Pseudomonas putida (H 18). The optimal conditions for biodegradation were observed in media containing phenanthrene and pyrene as sole carbon source, yeast extract as nitrogen source, and 4% of inoculum size, at pH 8 and 35ºC under static condition for 96 hrs. The maximum biodegradation efficiency was reached to 92% of phenanthrene and pyrene and was confirmed by using GS-mass spectroscopy.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
24
26
Invitro study for the optimal production of Hepatitis B Virus Subviral Particles for medical purposes
Reda Abdul Aziz Suef , Ezz Elden Mahmoud Mohamed , Mohammed Tarek Mansour , Mohamed Mansour Saad Farag
Assistant lecturer in Microbiology, Botany & Microbiology Department, Faculty of Science, Al-Azhar University
Associated prof in Microbiology, Botany & Microbiology Department, Faculty of Science, Al-Azhar University
Prof. of virology & Immunology, Faculty of Medicine, Cairo University
Associated prof. in Virology & Immunology, Botany & Microbiology Department, Faculty of Science, Al-Azhar University
ABSTRACT
During hepatitis B virus (HBV) replication, HBV infected cells release infectious viral particles and large amounts of subviral particles that are assembled by the surface protein without the viral genome. In the present study, HepG2.2.15 cell lines genetically integrated with the HBV surface region was cultured as an expression system for in vitro production of hepatitis B virus subviral particles (HBVsvp) at different time for commercial purposes. Our findings showed that culturing and incubation of HepG2.2.15 cells over 7 days incubation period resulted in continuous secretion of HBsvp in the supernatant and quantitative detection of HBsAg by ELISA indicated that the highest virus titer was at day 7. According to their size, subviral spherical particles recognized by a diameter of 22 nm. In conclusion, in vitro cultivation and incubation of HepG2.2.15 for secretion of HBsAg may be used in studying HBV replication thus, opening a new era for various studies from basic virology, in vitro diagnosis, to drug development against HBV genotypes.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
25
26
Advanced trends for production of Hepatitis B virus Subviral Particles using different technique to enhance overcome of Hepatitis B virus infection
prof. of virology at faculty of science Botany and Microbiology department Al-Azhar Univrsity
Prof. of virology&Immunolgy . National Cancer Institute . cairo
Associated Prof. of virology at faculty of science Botany and Microbiology department Al-Azhar Univrsity
ABSTRACT
Hepatitis B virus (HBV) infection is one of the leading risk factors for chronic hepatitis, liver fibrosis, cirrhosis and hepatocellular cancer (HCC). which are a major global health problem even with vaccine use and self-resolution in most cases. Hepatitis B virus (HBV) contains 3 types of particles, i.e., 22-nm-diameter spherical and tubular sub viral particles (SVPs) and 44-nm-diameter Infectious Dane particles. Both of spherical and tubular subviral particles are non-infectious and present strong immunogenicity. The main goal of this study was to optimize the best route for production of subviral particles that released from integrated HepG2.2.15 cell line in vitro. To achieve this aim, integrated HepG2.2.15 cell line was cultivated for production and concentration of SVP. The SVP were examined by serological marker and electron microscopy. Our results indicated that cultivation of HepG2.2.15 cell line in complete Williams medium E including Heat inactivated fetal bovine serum (FBS), insulin and hydrocortisone results in production high amounts of secreted viral particles in the supernatant. For the morphogenesis and structures of SVP, electron microscopy showed different spherical and filaments shapes for SVP sharing the same morphology of HBV virion while t it’s completely different in the diameter. These findings shed light on an important technique used in Production of huge number of HBVSVP which is very important step in the HBV infectious cycle, as the accumulation of HBV sub viral particles may be directly linked to viral pathogenesis in order to enhance overcome of HBV infection.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Microbiology and it's applications
26
26
Optimum Conditions for Production, Purification, Characterization and Application of Xylanase Produced by Trichoderma viride (RCMB) 017002 Under Solid State Fermentation
Ayman A. Farrag , Mohammed Hasan El-Haw , Ahmad Abd El-Kader Al-Bokhomy
Botany and Microbiological Dept., Faculty of science (Boys), Al-Azhar University, Cairo, Egypt
Botany and Microbiological Dept., Faculty of science (Boys), Al-Azhar University, Cairo, Egypt
Al-Azhar University, Tanta, Egypt
ABSTRACT
In this study, Trichoderma viride was selected to grow under solid state fermentation for xylanase production. The maximum xylanase production was achieved when the medium was supplemented with sugar beet pulp as a carbon source, the activity was 330.3 U/gds. The most potent nitrogen source was yeast extract as organic source and ammonium sulfate as inorganic source which gave xylanase activities 330.3 and 328.6 U/gds respectively. Maximum xylanase production took place at inoculum size 1.8×105 spores/ml which determined by 330.3 U/gds. Xylanase production increased as the incubation period increased and reached its maximum activity in the eighth day of the incubation with enzyme activity 330.3 U/gds. The maximum xylanase production occurred at pH 5.5 with enzyme activity 330.3 U/gds. Xylanase production increased gradually by the rise in incubation temperature and reached its maximum production at 30 ºC which represented by 330.3 U/gds. The highest level of xylanase production was observed upon addition of 0.1% tween 40, the activity was 330.3 U/gds. Trichoderma viride spores also were irradiated with increasing doses of gamma–rays. The maximum xylanase production was observed upon using 0.7 kGy of gamma irradiation doses which the activity was 650.3 U/gds as compared to the parent strain (un-irradiated). Mixed cultures of Trichoderma viride and Penicillium janthenellum used for xylanase production and increased the xylanase degrading capability by about 11% higher than Trichoderma viride only. The results showed that, the purification of xylanase was done by the conventional methods of centrifugation followed by ammonium sulfate. The purified enzyme was active and stable at all pH ranges, it increased gradually until reached maximum activity 17.24 U/ml at pH 6, then decreased gradually. The purified enzyme exhibited maximum activity at 40 ºC which represented by 17.24 U/ml and remains stable up to 50 ºC. There was increase in the purified β‐xylosidase enzyme activity by using Ca+2. The results showed that, mixed crude enzymes of xylanase and cellulase facilitated lignocellulose saccharification by improving lignocellulosic substrate accessibility higher than xylanase only.
