Recent trends in human affairs and marine environment, with a prospective on the Egyptian marine and fisheries resources
أ.د./ مصطفى مختار فودة
أستاذ علوم البحار ومستشار وزير البيئة - Minister Adviser on Biodiversity Ministry of Environment
ABSTRACT
This review is based on 1) World Ocean Assessment (WOA); 2) First Census of Marine Life 2010 (Highlights of a Decade of Discovery); and 3) Personal experience gained in the marine affairs. WOA includes more than 50 subjects within 4 main themes: marine environment and understanding of the ocean`s role in the global integrated Earth system; food security and food safety; human activities and their impacts; and marine biodiversity. The decade of discovery includes 540 expeditions, 2, 7000 scientists from more than 80 nations to assess and explain the diversity, distribution, and abundance of marine life, and points to opportunities to discover new species. Personal experience in the marine affairs includes knowledge gained nationally (Red Sea and Mediterranean Sea), regionally (Arabian Gulf, Arabian Sea and Gulf of Oman) and internationally (UN Convention on the Law of the Sea, Convention on the Biological Diversity, RAMSAR (Wetlands), Convention on the Migratory Species, and Convention on International Trade on Endangered Species). The main findings and conclusions are:1) climate change and related changes in the atmosphere have serious implications on marine ecosystems; 2) exploitations of the marine resources has exceeded sustainable levels in many regions; 3) fish products are the main source of animal protein; 4) there are clear gradients in biodiversity around the world;5) increased levels of use of marine resources create conflicting demands; 6) increased levels of population and industry result in increasing input of harmful material and excess nutrients in the marine environment; 7) adverse impacts on biodiversity come from the cumulative impacts of human activities; 8) the distribution around the world of the benefits drawn from the marine environment is still very uneven; 9) sustainable use of marine resources cannot be delivered unless integrated management of different sectors is achieved, and 10) the greatest threat is that of delay in implementing known solutions.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
.::. Invited Lecture
2
46
محاضرة مدعوة
أ.د./ وحيد محمود امام
أستاذ البيئة بعلوم عين شمس– رئيس الجمعية الأكاديمية المصرية لتنمية البيئة– أمين عام منتدى العلماء العرب - كلية العلوم جامعة عين شمس
ABSTRACT
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
.::. Invited Lecture
3
46
Fish Production in Egypt: Current Status, main challenges, and Future Perspective
أ.د./ سحر فهمي مهنا
أستاذ بيولوجيا المصايد بالمعهد القومي لعلوم البحار والمصايد ومدير فرع السويس سابقاً - المعهد القومي لعلوم البحار والمصايد
ABSTRACT
Egypt drives its fish yield from two main resources; wild or natural resources and artificial or aquaculture. The natural fisheries resources in Egypt classified into marine (Red and Mediterranean seas) an inland (lakes and River Nile) resources. These fisheries are one of our chief sources of wealth. If we give them due care, we can increase our national income and solve many of our problems. Despite the improved fish production in Egypt (≈1,8 million ton in 2017: about 20% from capture fisheries and 80% from cultured fish), the increasing pressure from growing populations and the desire to increase per capita consumption of fish requires us to reorder priorities in order to achieve optimum exploitation of available fish resources. Egypt as most of the world needs to focus on fisheries, as productive renewable food resources that can contribute to the development or increase national income as well as save the food for coming generations. Generally, Egyptian fish stocks from marine and inland waters have severely declined in recent years. The decline of the fisheries has stressed the need for efficient fisheries management. To develop the fisheries sector in present-day, Egypt’s attention must be paid to the development of its natural fish resources through better conservation and management of marine and fresh-water resources. So, this presentation will focus on the current situation of our natural resources and summarize the main challenges faced by this important sector as well as suggesting some applicable solutions to overcome these challenges and conserve our resources.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
4
46
Dromedary camel epididymal sperm characteristics at breeding and non-breeding seasons
Elsharnoby, H. A , Kandil, O. M. , Abu-Elnaga, N. A.
Embryology, Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls)
Embryology, Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls)
Embryo Biotechnology, Department of Animal Reproduction & Artificial Insemination, Veterinary Research Division, National Research Centre
ABSTRACT
A total number of 30 clinically healthy male dromedary camels were used in the present study. The age of these camels ranged from 5 to 10 years. Semen was collected twice a month for a year. The experimental work aimed to investigate the effect of seasonality on dromedary camel epididymal sperm characteristics (motility, viability, sperm cell concentration, morphology and membrane integrity). The obtained results showed that, the percentage of sperm motility, sperm-cell concentration, live sperm, normal sperm morphology, and the sperm with intact membrane were significantly (P<0.05) higher during breeding (November to April) than non-breeding (May to October) season, while the percentages of dead spermatozoa, sperm abnormalities and the damage membrane of spermatozoa were significantly (P <0.05) higher during non-breading as compared to breeding season. In conclusion, the results of the study has clearly indicate there are effects of seasonality on the reproductive capacity of male dromedary camels.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
5
46
Morphological and molecular characterizations of two Acanthamoeba spp. in drinking water in El–Menofia Governorate, Egypt
Yasmen A. Abdelaziz , Ahmed Abd El-Aziz , Wafaa F.A. Ahmed , Mohamed F. Abou El-Nour , Ahmad Z. Al-Herrawy
Biological and Environmental Sciences Dept., Faculty of Home Economics, Al-Azhar University, Egypt
Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, 11884, Cairo, Egypt
Biological and Environmental Sciences Dept., Faculty of Home Economics, Al-Azhar University, Egypt
Zoology Department, Faculty of Science, Al-Azhar University, Nasr City, 11884, Cairo, Egypt
Environmental Parasitology Lab., Water Pollution Research Dept., National Research Centre, 12622, Dokki, Giza
ABSTRACT
Providing suitable drinking water for human use is a major goal in all countries and contamination of drinking water with microorganisms represents a great danger to human health. Free living amoebae (FLAs) are found in all natural and manufactured environments, including aquatic environments. Some FLAs such as Acanthamoeba spp. infect the central nervous system of human with granulomatous amoebic encephalitis (GAE). The purpose of this investigation was to search about FLAs in drinking water in El-Menofia Governorate and to morphological study the most prevalent FLAs species, which were two Acanthamoeba pp. Molecular study was carried out to confirm the identification to the species level using a simple PCR technique.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
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مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
6
46
BIOCHEMICAL COMPOSITION OF SOME BIVALVES FROM THE NORTHWESTERN COASTS OF THE SUEZ GULF, EGYPT
Hosam A. M. El Mekawy , Awaad A. M. El-Sayed , Mohamed A. Amer , Mostafa H. Sarhan
Faculty of Science, Al-Azhar University, Cairo, Egypt.
Faculty of Science, Al-Azhar University, Cairo, Egypt.
Faculty of Science, Al-Azhar University, Cairo, Egypt.
Faculty of Science, Al-Azhar University, Assiut Branch, Egypt.
ABSTRACT
Bivalves are important components in the aquatic environment. Their consumption has been increasing worldwide during the past decades. The study of biochemical composition became much more important from the nutritional point of view. So, this study was conducted to estimate the percentages of edibility, total protein, total lipid and total carbohydrates in the most common and economically important bivalves, Mactra olorina (Family: Mactridae), Callista florida, Circe rugifera and Gafrarium pectinatum (Family: Veneridae) collected from the northwestern coasts of the Suez Gulf, Egypt. The results of these parameters showed remarkable seasonal variations. Edibility was higher in M. olorina compared with lowest value in C. rugifera. The highest annual values of total protein recorded 8.58±2.25 % and 8.19±3.87 % for C. rugifera and C. florida respectively; but the values of total lipid were low and recorded annual values of 5.38±3.49% for C. florida and1.90±1.64% for M. olorina. In contrast, the annual values of total carbohydrates were the higher, recorded 23.46±11.98% in C. florida, and 9.16±10.38% in G. pectinatum. Six amino acids (Tyrosine, Asparagine, Phenylalanine, Lysine, Arginine and Tryptophan) were estimated in M. olorina and C. florida. Tyrosine was the dominant with highest percentages of 20.75 and 15.09, in the two species respectively.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
7
46
Synchaeta (Rotifera) as bio-indicators for some pollutants at Al-Max Bay, Alexandria, Egypt
Mohammad S. Abu Husein , Khaled A. El-Damhougy , Awaad A. El-Sayed , Sawsan M. Aboul Ezz
Zoology Dep., Faculty of Science, Al-Azhar University
Zoology Dep., Faculty of Science, Al-Azhar University
Zoology Dep., Faculty of Science, Al-Azhar University
National Institute of Oceanography and Fisheries
ABSTRACT
Synchaeta community has been studied seasonally at Al-Max Bay during the period from spring 2014 to winter 2015. The study throws light to potential of some Synchaeta species to be bioindicators for some pollutants. Eleven Synchaeta species were recorded through this study at Al-Max Bay. The most frequent and dominant species were Synchaeta oblonga which dominated at all studied sites, and then Synchaeta okai which dominated at sites of II, III and IV, followed by Synchaeta pectinata which dominated at site III only. These dominant species correlated positively with salinity, dissolved oxygen, pH and nitrite, and thus they consider as bioindicators for these environmental parameters. So Synchaeta species can be used as bioindicators.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
8
46
POTENTIALITY OF COPEPODA (CRUSTACEA) AS BIO-INDICATORS FOR CERATAIN POLLUTANTS AT AL-MAX BAY, ALEXANDRIA, EGYPT
Khaled A. El-Damhougy , Mohammad S. Abu Husein , Awaad A. El-Sayed , Sawsan M. Aboul Ezz
Zoology Dep., Faculty of Science, Al-Azhar University
Zoology Dep., Faculty of Science, Al-Azhar University
Zoology Dep., Faculty of Science, Al-Azhar University
National Institute of Oceanography and Fisheries, Alex
ABSTRACT
Copepoda Community was studied seasonally at Al-Max Bay from spring 2014 to winter 2015. The study throws light on the potentiality of Copepoda species to be bio-indicators for certain pollutants and environmental factors. Thirty three species belong to 21 genera in addition to copepodites and nauplii were recorded during this study. Copepoda exhibited significant positive correlation coefficients with salinity in spring and autumn (r = 0.615 and 0.792 respectively) and with COD in summer and winter (r = 0.601and 0.709 respectively), in addition to temperature and phosphate in winter (r =0.672 and 0.645) and pH in autumn (r = 0.728). The highest similarity was noticed between the offshore sites III and IV. The dominant species of Copepoda were Oithona nana (at all sites) and Cyclops vernalis (at sites I and II) in addition to nauplii and copepodites. Oithona nana correlated positively with salinity, dissolved oxygen, pH in spring, summer and winter, and with phosphate and nitrite (agricultural effluents) in autumn, so it considers bio-indicator for these conditions. While Cyclops vernalis correlated positively with Escherichia coli, COD and phosphate in winter. Thus Cyclops vernalis can be used as bio-indicator for sewage pollution (Escherichia coli), industrial pollutants (COD) and agricultural effluents (phosphate).
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
9
46
REPRODUCTIVE BIOLOGY OF THE CO-EXISTING TRUE CRABS, PACHYGRAPSUS MARMORATUS (GRAPSIDAE) AND, ERIPHIA VERRUCOSA (ERIPHIIDAE) FROM THE EGYPTIAN MEDITERRANEAN SEA, ALEXANDRIA, EGYPT
El-Sayed, A. A. M. , AL_ Damhogy, Kh. A. , Fouda, M. M. , Salama A. F
Zoology Department, Faculty of Science (Boys) Al-Azhar University
Zoology Department, Faculty of Science (Boys) Al-Azhar University
Consultant in the Egyptian Environmental Affair Agency (EEAA), Cairo, Egypt).
Environmental Researcher in EEAA, Alexandria Branch
ABSTRACT
The present study provides detailed information on the reproductive biology of the Marble rock crab, Pachygrapsus marmoratus (Family Grapsidae) and the Warty crab, Eriphia verrucosa (Family Eriphiidae), collected from Alexandria coastline (Mediterranean Sea) during the period from June 2011 to May 2012. A total of 497 specimens of P. marmoratus and 122 of E. verrrucosa were collected monthly from the rocky intertidal and shallow subtidal zones at Bir Massud, Sedi Bisher and National Institute of Oceanography and Fisheries. Mature males and females of P. marmoratus and E. verrucosa were distinguished externally by the abdominal shape, which varied from semi-circular to rounded provided with four pairs of biramous abdominal appendages in females compared with narrow or tapering-shaped, with two unequal pairs of pleopods, the first is the longer in males. The overall sex ratios recorded 1: 0.87 and 1:0.90 males: females for P. marmoratus and E. verrucosa populations, respectively, and showed monthly and seasonal significant variations (P< 0.05). Based on the appearance of berried (gravid) or ovigorous females, both species has a lengthy definite breeding season starts in March and extends to late August for P. marmoratus and September for E. verrucosa. The frequencies of ovigerous females increased gradually and reached 100 % in May 2012 for P. marmoratus and in June 2011 for E. verrucosa; but were disappeared completely during the period from September and October to the following February for the two species, respectively. The diameter of incubated eggs ranged between 152 and 237.5 m in P. marmoratus, and from 332.5 to 503.5 μm in E. verrucosa and showed remarkable color changes during the different developmental stages. The egg number (F) of ovigerous females varied from 18,879 to 292,150, averaged 129,458 ± 56,416 eggs /female for P. marmoratus, and from 16,783 to 341,773, averaged 163,434 ± 194,401eggs /female for E. verrucosa. There were positive logarithmic relationships between carapace length (CL) and total egg number (F) for ovigerous females of both species (P< 0.05).
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
10
46
CHARACTERIZATION SPATIAL AND TEMPORAL VARIATIONS OF THE COMMON MACRO BENTHIC INVERTEBRATES IN THE GULF OF AQABA PROTECTORATES, RED SEA, EGYPT
Kareem F. Darweesh , Ahmed M. Hellal , Amr F. Zeina
This study focuses on temporal and spatial variations of macro benthic invertebrates composition, distribution, and diversity in the protectorates of the Gulf of Aqaba (Ras Mohammad, Nabq and Abu Galoum). Nine designated sites along of the Egyptian Coast of the Gulf were studied seasonally during the period from January to November, 2015. In total, 148 species belonging to 3 phyla, 13 classes, 25 orders, 66 family and 114 Genera were recorded. Phylum Mollusca showed the highest diversity and represented by 90 species (60.8 %). Echinodermata comprised 28 species (18.9 %), then Crustacea represented by 27 species (18.3%). Other taxa included (Cnidaria, Platyhelminthes and Annelida) each represented by only one class and one species.
Regarding to Spatial distribution, Abo Galoum Protectorate showed the highest diversity with 110 species, Ras Mohammed comprised 101 species and finally Nabq Protectorate with 93 species, among all protectorates phylum Mollusca showed the highest diversity, then Echinodermata and Crustacea came last. Concerning with the temporal distribution, Abo Galoum had the highest during the autumn season; however, the highest diversity recorded during autumn and winter seasons at Nabq Protectorate. Finally at Ras Mohammed the highest diversity of species recorded during summer season.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
11
46
Mixing pattern and physiological behavior of B. alexandrina exposed to Profenophos and Sesbania sesban mixed with different ratios.
Amr F. Zeina , Khaled A. El-Damhougy , Hossam M. Hwihy
Ass. Prof. Marine invertebrates Fac. Of Science AlAzhar university
Prof. Of Invertebrates Faculty of Science Alazhar university.
Ass. Lecturer, Faculty of Science, Alazhar university.
ABSTRACT
The present work aimed to study the mixing pattern (combination index and poly genograms) at short term (96H of exposure) and physiological behavior (total proteins, T. carbohydrate, T. lipids, ALT and AST) at long term of Biomphalaria alexandrina that exposed to Profenofos (PFF) and methanol extract of Sesbania sesban (MESS) in combinations with ratios: 1:9, 1:3, 1:1, 3:1 and 9:1 (PFF:MESS) respectively. The results showed that all treatments revealed antagonistic effect (CI value) varied from 1.62 to 4.32. Continuously exposure of B. alexandrina to LC0 of PFF and MESS combinations for 4 weeks caused significant reduction of total proteins, total carbohydrate and total lipids while AsT and AlT enzyme were significantly elevated. All survived snails had significantly recovered through two weeks.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
12
46
EFFECT OF SOME ADDITIVES TO FISH DIETS ON GROWTH PERFORMANCE OF NILE TILAPIA, OREOCHROMIS NILOTICUS
The present work aimed to evaluate the effect of nutritional plant additives (Moringa, Azolla and the mixture (fenugreek+ garlic+ spinach) on growth performance of Nile Tilapia, Oreochromis niloticus. In an experiment, 280 fish were divided into 7 groups in 14 plastic aquaria (60 x 50 x 40 cm) with stocking density of 20 fish / aquarium (2 replicate aquaria were assigned for each experimental group). Fish groups were fed for 3 months with different feed rations (C: plain food; T1: 5% Azolla; T2: 10% Azolla; T3: 5% Moringa; T4: 10% Moringa; T5: 5% mixture and T6: 10% mixture).
Results showed that, the highest average of growth performance of O. niloticus, were recorded when the fish fed on diet containing 10% mixed of fenugreek + garlic + spinach (T6). Also, the second greatest average of growth performance of O. niloticus was recorded in the fish fed on this diet (T6). The second highest average of growth performance of O. niloticus was recorded in the fish fed on diet containing 10% Azolla (T2). The greatest averages of growth performance in weight parameters (W, WG, DWG, G in W and SGR) of Oreochromis niloticus were recorded in the fish fed on diet containing 10% Azolla (T2). The third greatest growth in weight of O. niloticus was recorded in the fish fed on diet containing 5% Moringa (T3).
Data showed that, the lowest averages for growth performance in length and weight parameters of O. niloticus were recorded in fish fed on diet containing 5% mixture of fenugreek + garlic + spinach (T5). The second lowest averages for all growth performance parameters of O. niloticus were recorded in control group and T4 (diet containing 10% Moringa). The best food conversion ratio (0.92) was recorded in T6 (diet containing 10% mixture of fenugreek + garlic + spinach), followed by T3 and T2 (0.95 and 0.99, respectively).
In conclusion, the growth performance parameters of O. niloticus were improved when the fish fed on diet containing 10% mixture of fenugreek + garlic + spinach (T6); when fish fed on diet containing 10% Azolla (T2) or when fish fed on diet containing 5% Moringa (T3).
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
13
46
EFFECT OF REPLACING THE FISH MEAL BY PLANKTON BIOMASS ON GROWTH PERFORMANCE OF SEA BASS, DICENTRARCHUS LABRAX (LINNAEUS, 1758)
Ahmad Mosad Azab , Hamdy A. Abo-Taleb , Mohamed M.M. El-feky , Salama E. El-Nagar
This study aims to evaluate the effect of replacing the fish meal by plankton biomass on the growth performance of sea bass, Dicentrarchus labrax (Linnaeus, 1758). A total of 900 fries of the Dicentrarchus labrax (Mean body weight of 147.78mg ±15.63) were collected from fish hatchery Kilo 21, Alexandria and transported to the fish rearing unit in El-Max Research Station, National Institute of Oceanography and Fishers (NIOF), Alexandria, Egypt. After two weeks for acclimation, the fish were divided into 5 groups, 60 fish each, with 3 replicates for each group. Consequently, the fish were randomly distributed to 60 fish per cubic meter of haba in a concrete tank. The haba were filled with saline water in which oxygen saturation is 5.6 g/l at pH 7.9. Water temperature range was 26-27 °C. The five diets; group 1 (G1) that fed on a commercial pellets diet; other 4 groups fed on this commercial pellets diet with replacing fish meal by plankton biomass {group 2 (G2) 25% zooplankton; group 3 (G3) 50% zooplankton; group 4 (G4) 75% zooplankton and group 5 (G5) 100% zooplankton}. The feeding rate was 7% of the biomass. It was suggested that the positive effect of G5 in sea bass diets under the present study conditions may be due the release of growth factors. From the economic point of view the use of zooplankton (in G5) for Dicentrarchus labrax also increases their growth and production under farming conditions.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
14
46
ASSESMENT OF HEAVY METALS CONCENTRATION IN WATER, SEDIMENT AND EDIBLE MUSCLES OF NILE TILAPIA (OREOCHROMIS NILOTICUS) CULTIVATED IN DIFFERENT FISH FARMS IN EGYPT
Ahmad Mosad Azab , Hamdy A. Abo-Taleb , Ahmed Nasr Alabssawy , Zakaria Ibrahim El-Khateeb
The present work aimed to study the effect of water quality on the fish edible muscles and growth performance of Nile Tilapia, Oreochromis niloticus. Specimens of water, sediment and fish were obtained from private fish farms in Elsharkia governorate (Al-Abassa which supply with low polluted water and Bahr El-Baqar which supply with high polluted water). The concentrations of heavy metals (Cu, Zn, Fe and Pb) in water, sediment and edible fish muscle of Nile Tilapia, O. niloticus were analyzed and recorded.
The results reported that there were higher levels of the analyzed heavy metals in O. niloticus obtained from Bahr El-baqar farm (Fe: 166.27µg/g, Zn: 121.53 µg/g, Cu: 29.91 µg/g and Pb: 1.82 µg/g) than that from Al-Abassa fish farm (Fe: 42.79µg/g, Zn: 50.30 µg/g, Cu: 15.17 µg/g and Pb: 0.21µg/g).
It is concluded that the fish obtained from Bahr El-baqar fish farm may be not completely safe for human consumption, when compared to the World Health Organization (WHO) standards.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
15
46
Survey of Reptiles in Nechisar National Park, Southetn Ethiopia.
Zoology Department, Faculty of Science, Al Azhar University, Cairo, Egypt.
Department of Integrative Biology, Oklahoma State University, Stillwater, OK, United States of America
Zoological Sciences Program Unit, College of Natural Sciences, Addis Ababa University, Addis Ababa, Ethiopia
ABSTRACT
Studies on species composition, diversity, evenness and relative abundance of reptiles in Nechisar National Park were carried out from October 2007 to April 2008. Reptiles were surveyed using active search, pitfall trapping and cover sheet methods. A total 34 species belonging to 3 orders (Order Squamata, Order Testudines and Order Crocodylia) were recorded. Order Squamata was the most diverse containing 30 species belonging to 2 Suborders, Sauria which comprises 19 species belonging to 7 Families and 10 Genera and Serpentes comprising 12 species belonging to 4 Families and 13 Genera. Order Testudines comprised 3 species belonging to 2 Families. Order Crocodylia comprised only one species. The diversity of reptiles varied among the different habitat types. Bushland habitat had the highest number of reptilian species (29.69% of the collected species). From the total species recorded 11 (29%) were new records from the area.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
16
46
CULTURING OF OREOCHROMIS NILOTICUS MONOSEX FINGERLINGS AT DIFFERENT DENSITIES USING A BIOFLOC TECHNOLOGY.
Hossam M. Hwihy , Amr F. Zeina , Khaled A. Eldamhougy
Ass. Lecturer, Zoology dep. Faculty of Science, Alazhar university Cairo
Ass. Professor, Zoology dep. Faculty of Science, Alazhar university Cairo
Professor, Zoology dep. Faculty of Science, Alazhar university Cairo
ABSTRACT
A 20-week experiments were conducted to evaluate the effect of Biofloc technology (BFT) on Oreochromis niloticus mono sex fingerlings, focused on water quality, growth parameters, Hematology, Biochemistry, body composition and somatic indexes. Duplicated treatments were designed, two Biofloc treatments with two densities (12 &16 fish/200 L tank) vas two control groups. All treatments were fed with 3% of body weight in two equal divided meals. Rice brane and Molasse (1:1) were added to Biofloc treatments to establish a carbon/nitrogen (C/N) ratio of 15:1 twice daily after one hour of meals. Biofloc treatment were significantly decrease TAN and Nitrite levels. Biofloc treatments showed significantly high average final body weight, better feed conversion ratio (FCR) and one ninth water consumption. Biofloc addition had generally no significant difference of body composition, hematology, biochemistry and Hepatosomatic (HSI), while Spleenosomatic index (SSI) was significantly increase at high density only. It could be concluded that Biofloc treatments improve water parameters stability and fish growth performance While it decreased water consumption. High density (80fish/m3) is more yield productive treatment.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
17
46
Comparative Histological and Histochemical Studies on the Esophagus of Siluroid Fishes, Clarias gariepinus and Bagrus bajad Inhabiting Bahr Shebeen-Alkoom, Al-Menoufiya Governorate, Egypt
Sabry M. A. Shehata , Ahmad M. Azab , Diaa M. G. Farrag
Zoology Department, Faculty of Science, Al-Azhar University, Cairo
Zoology Department, Faculty of Science, Al-Azhar University, Cairo
Zoology Department, Faculty of Science, Al-Azhar University, Cairo
ABSTRACT
The present work aimed to describe and compare the histological and histochemical structures of the esophagus in both of the omnivorous fish, Clarias gariepinus, and the carnivorous fish, Bagrus bajad. For this purpose, 20 specimens of esophagus from the two species were collected from the River Nile at Bahr Shebeen Canal at Shebeen-Alkoom city, Al-Menoufiya governorate, Egypt.
Histologically, results revealed that the esophagus of the two species is made up of the four layers: mucosa, submucosa, muscularis, and serosa. The esophageal mucosa lined by stratified epithelium; The epithelial mucosa of both fishes is made up of stratified epithelium, the club-cells were either present in only one or two layers of the epithelium in C. gariepinus or scattered all over the entire stratified epithelium in B. bajad. Mucous cells are highly abundant. The mucus cells contain mixed of acid and neutral MPS in the esophagus of omnivorous fish, C. gariepinus while contain neutral MPS in B. bajad. The esophagus is concerned with mucus production, to protect the mucosa against chemicals, parasites, hypertonic media and acidity, as well as to provide lubrication for the food toward the stomach.
In conclusion, the obtained differences in the structures of esophagus in both fish species may be related to their different feeding habits and type of food.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
18
46
MORPHOLOGICAL ASPECTS OF CAUDAL FIN FOR EIGHT SHARK SPECIES WITH SPECIAL REFERENCE TO THE FIRST RECORDS IN EGYPTIAN MEDITERRANEAN WATER, ALEXANDRIA, EGYPT
Ahmad Mosad Azab , Hassan Mashhout Mohamed Khalaf-Allah , Moustafa M. H. Sarhan , Mohamed A. M. El-Tabakh
The present study aimed to describe the caudal fin measurements used to differentiate between some shark species in Egyptian Mediterranean water. A total of 43 specimens of sharks were seasonally collected from the commercial catch of the Mediterranean Sea, at land fish markets in Alexandria, during the period from May 2017 to June 2018. Morphometric characters of caudal fin were recorded for each specimen. Many photos had been captured for each shark specimen and processed by Image J software to calculate different ratios of morphological aspects for caudal fin.
Results showed that the collected specimens belong to 8 species (Heptranchias perlo, Hexanchus griseus, Squalus megalops, Centrophorus uyato, Oxynotus centrina, Squatina squatina, Isurus oxyrinchus and Isurus paucus) and three species of them (Heptranchias perlo, Squalus megalops and Isurus paucus) are new records in the Egyptian Mediterranean water. The morphological aspects of caudal fin in these eight shark species were greatly varied in shape. The statistical analysis of morphometric ratios showed significant variances between investigated species. Our study attempted to add more update information on shark caudal fin morphological and dimensional scaling.
The present study concluded that the caudal fin morphological aspects of studied shark species proved the potential capability of this method for shark species identification.
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CSC
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
19
46
BODY MORPHOLOGICAL ASPECTS FOR EIGHT SHARK SPECIES WITH SPECIAL REFERENCE TO CONSERVATION STATUS OF THEIR POPULATIONS IN EGYPTIAN MEDITERRANEAN WATER, ALEXXANDARIA, EGYPT
Ahmad Mosad Azab , Hassan Mashhout Mohamed Khalaf-Allah , Moustafa M. H. Sarhan , Mohamed A. M. El-Tabakh
The present study aimed to describe the morphology and morphometric measurements used to differentiate between some shark species in Egyptian Mediterranean water. A total of 43 specimens of sharks were seasonally collected from the commercial catch of the Mediterranean Sea, at land fish markets in Alexandria, during the period from May 2017 to June 2018. Morphometric characters of shark bodies were recorded for each specimen. Many photos had been captured for each shark specimen and processed by Image J software to calculate different ratios of the body morphological aspects.
Results showed that the collected specimens belong to 8 species (Heptranchias perlo, Hexanchus griseus, Squalus megalops, Centrophorus uyato, Oxynotus centrina, Squatina squatina, Isurus oxyrinchus and Isurus paucus) and three species of them (Heptranchias perlo, Squalus megalops and Isurus paucus) are newly recorded in the Egyptian Mediterranean water. The morphological aspects of the body of these eight shark species were greatly varied in shape. The statistical analysis of morphometric ratios showed significant variances between investigated species. Our study attempted to add more update information on shark body morphology and dimensional scaling.
The present study concluded that the body morphological aspects of the studied shark species proved the potential capability of this method for shark species identification.
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CSC
CHEM
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BIO
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
20
46
IDENTIFICATION KEY BY GILL RAKERS DIFFERENTIATION FOR SOME LABRID FISH, RED SEA, EGYPT
The present study aimed to identification of labrid fish species (Family: Labridae) inhabiting coral reef in Hurghada, Red Sea, Egypt based on differences in morphology of gill rakers as well as construction of a key to the identification of these species. Fishes were collected from land fish market in Hurghada of Egyptian Red Sea, during the period from April 2016 to May 2017. The gills of studied species were removed, stained and examined.
Results showed that the gill system in labrid fish species consists of four pairs of gills, which are termed from the lateral to medial as first, second, third and fourth gill arch. The first gill arch (outer) is the longest one. Each gill arch is bow-like shape; formed of one piece. It is displayed semilunar in shape, consisting of two limbs (upper and lower limbs). The gill arch carried gill rakers on its concave border and gill filaments on its convex one. Anterior and posterior rows of gill rakers are noticed on gill arches in all species of this study. The anterior row (oral row) and posterior (aboral row) of gill rakers varied in length and shape in the first gill arch; having long and more developed rakers in the first row and short with less developed in the second one. The number and shape of gill rakers is often used as a diagnostic character in species identification.
In conclusion: the differences in number and morphology of gill rakers can be used as diagnostic characters in identification of fish species in family: Labridae inhabiting coral reef in Hurghada, Red Sea, Egypt.
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CSC
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
21
46
Evaluation of the antitumor activity of some natural plant extracts on in vivo Ehrlich ascites carcinoma treated mice.
Zeinab Eid Madboly Hanafy , Rasha Aly Ahmed Aly El sayed , Hend Fouad Abd El Fattah , Asmaa Kutb Mohamed Amer
Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girl's), Cairo, Egypt
Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girl's), Cairo, Egypt
Pathology department, National Cancer Institut, Cairo University,Cairo, Egypt
Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girl's), Cairo, Egypt
ABSTRACT
In this study , the utility of three natural plats extract, Anastatica hierochuntica (AH), Lepidium sativum (LS) and Carcia papaya (CP) in the regulation and restoring the rate normal rate of cell death and cell division against in vivo Ehrlich ascites carcinoma (EAC) in female Swiss albino mice were tested. EAC was induced by intraperitoneal injection of EAC-cells in the female mice. EAC-bearing mice were orally treated with 500 mg/kg body weight of AH, LS and CP extracts for 7 and 14 days after EAC intraperitoneal transplantation. Animals are allocated in to nine groups, control group, oil, AH, LS and CP groups, EAC group (mice were inoculated with 2.5× 106 intraperitoneally (i.p), EAC+AH, EAC+LS and EAC + CP. Apoptotic cell death was determined in mice liver using florescent stains (Annexin V and propidium iodide) using flow cytometry, and mitotic index was investigated Geimsa stained slides of mice bone marrow cells to define and outline the degree of cell proliferation. Results revealed an increase in normal cell death mechanism accompanied by decreased mitotic index in EAC-bearing mice treated with the plants extracts that were more pronounced in the group with AH.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
22
46
" The influence of date on some hematological and biochemical parameters of albino rats exposed to venom .”
Bashandy1, M.A. , Abd-El-Aal2 ,A. , Ibrahim1,D.F. , El-Sharkawy1,M.A.
.Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
.Zoology Department, Faculty of Science, Ain-Shams University, Cairo, Egypt
.Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
.Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
ABSTRACT
The study was designed to determine the protective effects of date palm extract against Cerastes cerastes venom caused biochemical changes in the tissues of albino rats. Wister albino rats( 104 male rats) averaged weights (130±10g) at the beginning of the experiment were divided into 6 main groups according to the treatmen and requirements of the experiment. Control rats group, DPE group treated orally with DPE at dose 1.2 mg/kg body weight, venoms groups: Rats injected with a single dose of Cerastes cerastes for one day, this group divided into three subgroups injected by a single dose of 1/4 LD50, 1/2 LD50 and LD50 for one day also for two weeks groups, venoms treated with DPE groups treated orally with DPE for two weeks before /and after injecting of a single dose of venom at dose 1/4 LD50, 1/2 LD50 and LD50 for one day. Each group contains 6 rats except venom groups of LD50 were 8 rats and six rats were sacrificed after the 1st day, 2nd and 4th week from each group of
treatment. The results showed a significant raise of some hepatic parameters (ALAT, ASAT, ALP, TG, CHO and LDL-C), while a significant reduction in some other parameters (Total protein, albumin and HDL-C), on the 1st day and 2nd weeks in rats injected intraperitoneal with Cerastes cerastes venom as compared to the control groups. The administration of the DPE ameliorated the side effects of the poisonous changes of Cerastes cerastes venom.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
23
46
COMPARATIVE STUDIES ON THE INTEGUMENTARY SYSTEM OF CLARIAS GARIEPINUS AND BAGRUS BAJAD (ORDER: SILURIFORMES) IN NILE RIVER, EGYPT
Diaa M. G. Farrag , Ahmad M. Azab , Sabry M. A. Shehata
The present study aimed to describe the histological structures of the skin and compare between the structures in both of African catfish, Clarias gariepinus (Family: Claridae) and bagrid fish, Bagrus bajad (Family: Bagaridae). For this purpose, 20 specimens; 10 of African catfish and 10 of bagrid fish were collected from Bahr Shebeen Canal (a branch of the River Nile) at Shebeen-Alkoom City, Al-Menoufiya Governorate, Egypt. Histological changes in the skin in two species studied were photographed and described.
The absence of scales on the integument of C. gariepinus and B. bajad was the important characteristic feature to enable respiration through the skin. Both fishes are darker colored from above and lightly or whitish colored from below to camouflage itself from predators above and below. Results showed that the abundance of the mucus on the surface of the skin is correlated with the mode of life as it may serve to keep the skin clear for respiration. The mucus on the skin also may contribute a protective effect against pathogens and other surrounding environmental hazards. Histologically, the integument of both C. gariepinus and B. bajad consists of epidermis which essentially cellular in structure and dermis which is primarily a fibrous structure with relatively few cells. The middle layer of the epidermis in C. gariepinus and B. bajad is mainly composed of club cells and spherical mucous cells, in addition to vertically elongated epithelial cells.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
24
46
Biocontrol of exogenous stages of Eimeria stiedae (Apicomplexa: Eimeriidae)
1-Effect of some herbal medical plant extracts
Ahmed Abdel-Aziz Mohammed , Gamal M. Elsherbiny , Saad A.M. moghannem , Mohamed Fat-Halla Abo El-Noor , Hussein Abo El-fetouh Abd El-fadeel
Zoology Department, faculty of Science (Boy's) Al-Azhar University, Nasr City, Cairo, Egypt.
Plant & Microbiology Department, faculty of Science (Boy's) Al-Azhar University, Nasr City, Cairo
Plant & Microbiology Department, faculty of Science (Boy's) Al-Azhar University, Nasr City, Cairo
Zoology Department, faculty of Science (Boy's) Al-Azhar University, Nasr City, Cairo, Egypt.
Plant & Microbiology Department, faculty of Science (Boy's) Al-Azhar University, Nasr City, Cairo
ABSTRACT
In vitro anti-coccidial effect of four medicinal plant extracts (MPEs): clove Syzygium aromaticum, myrrh Commiphora molmol, olive Olea europaea and cactuses Aloe vera on Eimeria stiedae oocysts was evaluated. Un-sporulated oocysts were exposed to three different concentrations (w/v) (250, 500 and 1000 μg/ml) from each plant extract. Three replicates were made for each concentration. Sporulation inhibiting bioassay was used to evaluate the activity of MPEs on sporulation of eimerian oocysts. The results showed an anti-coccidial activity of MPEs against Eimeria stiedae oocysts as proved by its ability in not only inhibition as completely prevention of oocysts sporulation but also on destroying the oocysts. Inhibition of sporulation was observed in all concentrations of all MPEs. Also, myrrh extract damaged oocysts at all concentrations. At the highest dose, the destroyed efficacy of myrrh extract reached 91.00%, while inhibition of sporulation reached 100%. However, destroyed efficacy of clove, olive and cactuses extracts on eimerian oocysts reached 70.16%, 3.03% and 5.33%, respectively. Whereas, inhibition of oocysts sporulation of clove, olive and cactuses reached 23.72%, 75.21% and 100%, respectively. The study herein showed that the most potent plant extracts were cactuses and myrrh because of the sporulation was completely stopped by them.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
25
46
COMPARATIVE STUDY IN MORPHOLOGICAL AND HISTOLOGICAL ADAPTATIONS IN THE ESOPHAGUS AND STOMACH IN RELATION TO FEEDING HABITS OF SOME CORAL REEF FISHES AT HURGHADA, RED SEA, EGYPT
Walaa Tantawy Salama Shalaby
Zoology Department, Faculty of Science (girls), Al-Azhar University, Cairo, Egypt
ABSTRACT
The present study deals with the morphological and histological adaptations in the esophagus and stomach of some coral reef fishes (Parupeneus forsskali, Popilloculiceps longiceps and Acanthurus sohal) were adapted to feeding habits.
Results showed that, the esophagus of carnivorous fish, Parupeneus forsskali, piscivores fish, Popilloculiceps longiceps and herbivores fish, Acanthurus sohal were a short, narrow and distensible muscular tube to conduction of food. The great distensibility of the esophagus is adaptation for feeding. So a relatively large object can be swallowed.
The esophageal mucosa of carnivorous fish, P. forsskali is of a stratified type. The epithelial mucosa contains large mucus glands. The latters are large, highly abundant, tubular or elongated in shape and intermingled with much fewer of squamous epithelial cells.
The esophageal mucosa of piscivorous fish, P. longiceps and herbivores fish, A. sohal were thrown into primary folds and branched to secondary folds to increase of wide lumen during food consumption. The mucosa is of a stratified type. Mucus cells are highly abundant. Therefore, the food can easily pass posteriorly without causing any damage to the epithelial lining of the soft mucosa. The muscular coat is very thick and formed of one layer, only the circular. The strong musculature, elsewhere, however, would allow for distention during food consumption or increase the esophageal area for digestive activities.
The stomach of carnivorous fish, P. forsskali is of the sac-like shape. While, the stomach of piscivorous fish, P. longiceps is a great distensibility giving the bag-like shape to enable these fishes to take relatively huge prey. The stomachs in this species have a vast amount of gastric glands in the cardiac portion, while, in the pyloric one have a very thick layer of muscularis which helps in regulating the passage of the digested food to the intestine.
The stomach of herbivorous fish, A. sohal is U in shape. It can be divisible into cardiac and pyloric regions. Cardiac region is highly specialized as a masticator organ for the fish that eat hard vegetable matter. It is characterized by the tough non-cellular lining and a thick powerful muscle to form mechanical digestion. The epithelium of the pyloric stomach develops into a thin of columnar cells towards the lumen and a thick layer of gastric glands towards the base to form chemical digestion.
In conclusion, the esophagus and stomach of carnivorous fish, P. forsskali, piscivores fish, P. longiceps and herbivores fish, A. sohal are subjected to diverse and significant variations and much modification in accordance with the food type and feeding habits.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
26
46
Searching for sperm bio-activator from Egyptian scorpion venoms
Invertebrate lab., Zoology Dept., Faculty of Science, Zagazig University
molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
Invertebrate lab., Zoology Dept., Faculty of Science, Zagazig University
Invertebrate lab., Zoology Dept., Faculty of Science, Zagazig University
ABSTRACT
Searching for sperm bio-activator from Egyptian scorpion venoms
Background: Sperm motility is a key parameter for fertilization. Significant efforts have, therefore, been made to identify bioactive compounds that can improve sperm motility. In this study, we try to identify novel compounds that can reinforce sperm motility from the venom of four Egyptian scorpions; Androctonus australis, Androctonus bicolor, Leiurus quniquestratus and Scorpio maurus palmatus.
Materials and methods: Scorpions were collected, and venoms were milked using electrical stimulation. Serially diluted crude venoms at noncytotoxic concentration were examined on human sperm mobility. Different sperm motility parameters were measured In vitro with a computer-assisted sperm analysis (CASA) system in the presence or absence of the venom.
Results: We observed that the four studied scorpion venoms increased human sperm motility by increasing track speed (VCL) and lateral displacement of the head (ALH) with high selectivity index (SI). This increase was much higher than caused by pentoxifylline (PTX). Moreover, Venom treated with proteinase k completely lost its ability to increase sperm mobility. This implying that scorpion venoms are promising sources for bioactive molecules with proteinaceous nature that able to improve human sperm motility.
Conclusions: This report confirming that scorpion venoms contains sperm bioactiators. Bioassay-guided fractionation will be performed to characterize the candidate molecule.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
27
46
Chemical synthesis and biological activity of γ-conotoxin-like peptide isolated from the worm-hunting snail Conus fulgetrum
Zoology Department, Al Azhar University, Assuit, Egypt
Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Zoology Department, Al Azhar University, Assuit, Egypt
Zoology Department, Al Azhar University, Assuit, Egypt
Zoology Department, Al Azhar University, Assuit, Egypt
Graduate School of Agriculture, Kyoto University, Kyoto, Japan
ABSTRACT
Background: The novel conotoxin, named as fu6a, is a 26-residue cysteine-rich peptide belongs to the O2-superfamily containing two post-translationally modified residues a hydroxyproline (O) and gamma carboxyglutamic acid (γ). It was isolated from the crude venom of Conus fulgetrum by HPLC and mass spectrometry. Although fu6a represent one of the major components in the venom, its biological function remains unknown.
Methods: Here, fu6a and its analogues; fu6a without (Gla & Hyp), fu6a without (Gla) and fu6a without (Hyp) were chemically synthesized by using the Fmoc-based solid-phase peptide synthesis technique. To explore its biological function, a bioassay was performed for fu6a and its analogues by injecting the synthesized peptide independently into the snail Clithon retropictus foot muscle.
Results: In the present study, a novel conotoxin fu6a and its analogues were successfully synthesized by using the Fmoc-based solid-phase peptide synthesis technique.
Bioassay was performed for fu6a and its analogues by injecting the sample into the snail (Clithon retropictus, 300-550 mg body weight). Significant paralysis was observed 5 min post injection (0.25 nmol) of both fu6a and modified peptide containing γ-conotoxin only.
Conclusions: γ-carboxyglutamic acid (Gla) residue plays the major role in the peptide activity against snail. Moreover, fu6a plays an important role in defense against other snails. Finally, the structural similarity of peptide fu6a to other gama-conotoxins suggests that modulation of pacemaker channels could be responsible for its biological activity.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
28
46
Molecular mimicry with Giardia lamblia cysts showing the apparent occurrence of host- like antigen in rats
alaa abd el-aziz mohamed samn
Zoology Department , Faculty of Science , Al-Azhar University,Cairo , Egypt
ABSTRACT
The study included the role of cytokines in parasitic infections has been widely investigated in animal models, there is limited literature and few clinical works on the importance of Th1 (IL-2) and Th2 (IL-4, IL-10) in human giardiasis as well as the role of IgE antibody secretion during parasitic infections. Thus, the present work was undertaken to study the levels of these cytokines and IgE antibody in the sera of rats infected with G. lamblia. The presence of anti-parasitic antibodies detected in the serum depended on the secretion of cytokines such as IL-4 which is also produced from Th2 cells. The present study, presented evidence to support previous hypotheses regarding the existence of G. lamblia , and this variation may be related to differences in geographical origins and hosts. Moreover, rats infected with homogonic and heterogonic strains present differences in the time of infection. These results confirm the utility of studying G. lamblia infections in adult rats as a model of human giardiasis for understanding both pathogenesis and protective immunity against this infection.
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CSC
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
29
46
Ecological Studies on bivalve assemblage inhabiting seagrass beds in Red Sea (Hurghada, Egypt).
Walaa M. Shaban , Ahmed M. Hellal , Salah E. Abdel-Gaid
Zoology Department, Marine Biology and Fish Sciences section, Faculty of Science, Al-Azhar University, Cairo, Egypt
Zoology Department, Marine Biology and Fish Sciences section, Faculty of Science, Al-Azhar University, Cairo, Egypt
Zoology Department, Marine Biology and Fish Sciences section, Faculty of Science, Al-Azhar University, Cairo, Egypt
ABSTRACT
Bivalves found almost in all marine habitats and is extensively used to evaluate the health status of marine ecosystems. The present study was designed to evaluate the effect of seasonal conditions as well as seagrass habitat conditions on diversity and abundance of bivalves. Seagrass beds were selected to be dominated by the Red Sea native seagrass, Halophila stipulacea, which occur in sub-tidal waters of 2- 7 m depth. Bivalves ecological variables were measured seasonally in two sites: Hurghada Marine Station and Abou Monkar Island, during the period from mid-April 2016 to mid-January 2017. On the Other hand, seasonal floristic data were determined for seagrass species in both investigated sites to evaluate the regression value between floristic and faunal data. Overall, 29 bivalve species belong to 11 families were recorded. The seasonal variation in abundance fluctuated greatly among different families and species and classes. Data indicated that, species abundance showed the same pattern during the different seasons in both sites. The maximum average value of species abundance was 441 individuals at Abou Monkar Island during summer, while the minimum value was 76 individuals at Marine Station site during winter. Results indicated that, Mactra olorina were the highest abundance value being 29% of all bivalve recorded followed by Corbula sulculosa represented with 15 %.
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CSC
CHEM
GEO
BIO
ASM
PHY
10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
30
46
Spermicidal activity of venoms of common Arthropods from Egypt
1 molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
1 molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
1 molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
1 molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
2 Zoology Dept., Faculty of Science, Suez Canal University
3Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University
ABSTRACT
Background: Nowadays, hundreds of spermicidal products have
been marketed, containing a detergent as an
active ingredient. Such agents showed bad effect on epithelial cells and normal vaginal flora. They also increase the risk of vaginal or cervical infection and irritation or ulceration. Therefore, trials have been made to identify bioactive nontoxic and effective spermicides for vaginal contraceptives.
Objective: We aimed to elucidate the spermicidal activity of venoms of four common arthropods species from Egypt; Honybee; Apis mellifera, spider; Chaetopelma olivaceum, scorpions; Compsobuthus wereniri and Orthochirus innesi.
Materials and methods: Venoms were collected, serially diluted at noncytotoxic concentration and examined on human sperm mobility. Different sperm motility parameters were evaluated by the Sander–Cramer test under in vitro conditions in the presence or absence of the venom.
Results: Sperm motility was inhibited when treated with different arthropod venoms. The 100% effective concentration (EC100) was 3, 350, 300 and 24 ug/ml for bee venom, spider venom, scorpion venoms respectively. This implying that arthropods venoms are promising sources for bioactive molecules which able to inhibit human sperm motility.
Conclusions: This report confirming that arthropods venoms contains spermicidal agents. Bioassay-guided fractionation will be performed to characterize the candidate molecules.
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CSC
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
31
46
DNA BARCODING FOR THE SPIDER CRAB MENAETHIUS MONOCEROS (Latreille, 1825) FROM THE RED SEA, EGYPT
Mohamed A. Amer
Zoology department, Faculty of Science, Al-Azhar University, Cairo
ABSTRACT
Most spider crab species inhabiting the Red Sea has not been characterized genetically, in addition to the variation and complexity of morphological identification of some cryptic species. The present study was conducted to verify the identification of two morphs of spider crab Menaethius monoceros (Family Epialtidae Macleay, 1838) collected from Hurgada, Red Sea, Egypt. DNA barcoding partial sequencing of two markers mitochondrial cytochrome oxidase subunit I (COI) and ribosomal subunit (16s) were used to differentiate between these morphs. The genetic analyses revealed that there is individual variation within the same species. Therefore, DNA barcoding and genetic analyses with the morphology gave accurate identification of these morphs within the same species.
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CSC
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BIO
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
32
46
Immune stimulation and proapoptotic effect of Propolis and its conistituent Chrysin against γ-irradiated solid Ehrlich carcinoma bearing mice
Barakat E.M.S , Shouaib Z.A , Asmaa Z. Alsharkawy , Nermeen M Elbakary
Entomologyy Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Zoology Department, Faculty of Science, Al- Azhar University, Cairo, Egypt
Zoology Department, Faculty of Science, Al- Azhar University, Cairo, Egypt
3Radiation Biology Department, National Centre for Radiation Research and Technology, Atomic Energy authority, Cairo, Egypt
ABSTRACT
The increased interest in new approaches to the immunotherapy of cancer, and a considerable demand for therapeutic agents which can modulate the several forms of immunodeficiency have encouraged studies on the immunomodulatory mechanism of natural and synthetic substances . The present work was an endeavor to evaluate the imuno stimulating and pro apoptotic effect of Chrysin and Propolis on the tumor growth and the cell sensitivity to ionizing radiation targeting the improvement of cancer therapeutic protocols. Chrysin (20 mg /kg /day) and Propolis (100 mg/kg body weight/day) were injected intraperitoneal to mice bearing 1cm3solid tumor of Ehrlich ascites carcinoma (EAC) for 21 consecutive days. Mice were whole body exposed to 1 Gy of gamma radiation (two fractionated dose). Treatment with Chrysin or Propolis markedly suppresses the proliferation of tumor in EAC mice. The activity of IFN-γ was significantly decreased compared to EAC mice. The concentrations of m-RNA for angiogenic factors (TNF-α),free radicals as well as nitric oxide (NO) concentration were significantly decreased collimated with improvements in apoptotic regulators (Caspase-3 activitiy) compared to EAC mice. Moreover, the histopathological investigation confirms the improvement exerted by Chrysin or Propolis even in EAC mice group or EAC+R group. Exposure to gamma radiation sustained the modulatory effect of Chrysin or Propolis on tumor when compared with EAC+Ch or EAC+P mice. Hence, Chrysin or Propolis might represent a potential therapeutic strategy for increasing the radiation response of solid tumor.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
33
46
Cell free DNA concentration in spent embryo culture medium as a noninvasive molecular marker for embryo quality assessment
Mohammed Hesham , Ali Gadel-Rab , Moustafa Sarhan , Hesham Elshaer
molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University
ABSTRACT
Background:
Over the last decade, efforts in improving non-invasive embryo assessment have looked into the culture media of embryos to find a new noninvasive tool. Human embryos were found to release genomic DNA (gDNA) and mitochondrial DNA (mtDNA) into the culture medium. In this study we aimed to focus on cell free DNA (cfDNA) in culture medium as a noninvasive tool to monitor embryo development and quality.
Methods:
Day 5 embryos were collected and morphologically graded according to the current consensus system. cfDNA was extracted from each culture medium using QIAamp Circulating Nucleic Acid Kit. Simultaneously, human mitochondrial DNA was amplified using REPLI-g Mitochondrial DNA Kit. Extracted cfDNA and amplified mtDNA concentrations were measured to correlate between the concentration and grade of the embryo.
Results:
CfDNA was successfully purified from all tested embryo culture samples and confirmed by PCR (n=35). A significant correlation between cfDNA level and embryo grading was observed Notably, high levels of cfDNA were observed in poor embryo quality.
Conclusion: A significant correlation between cfDNA levels in spent culture media and embryo quality was observed. Therefore, cfDNA seems to be a promising a noninvasive tool to check embryo quality.
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CSC
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BIO
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
34
46
Antiviral activity of Egyptian cobra venom and it’s derivatives with a potent virucidal effect against hepatitis C virus and dengue virus
Zoology Department, Faculty of Science, Al-Azhar University, Egypt. - Department of Microbiology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Zoology Department, Faculty of Science, Al-Azhar University, Egypt.
Department of Microbiology, Kobe University Graduate School of Medicine, 7-5-1Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. - Department of International Health, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Suma-ku, Kobe 654-0142, Japan.
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194 Japan
ABSTRACT
Recently, growing global viral infections have been a serious public health problem worldwide. This current situation emphasizes the importance of developing more therapeutic antiviral compounds. Hepatitis C virus (HCV) and dengue virus (DENV) belong to the Flaviviridae family and are an increasing global health threat. Our previous study reported that the crude venom of Scorpio maurus palmatus and its peptide Smp76 possessed potent anti-HCV and anti-DENV activities in vitro. In this study, we evaluated the effect of the Egyptian cobra Naja haje venom and its derivatives on two Flaviviridae viruses HCV and DENV. The antiviral activity was evaluated using a cell culture technique utilizing Huh7it-1, Vero/SLAM, HCV (JFH1, genotype 2a) and DENV (Trinidad 1751, type 2). A potential antiviral activity of crude venom was detected in culture cells with an approximate IC50 of 0.1ng/ml. Moreover, the crude venom fractions possess potent virucidal activity against HCV and DENV. The crude venom and its derivatives of Egyptian cobra prevent HCV and DENV infection and suppress secondary infection, by inactivating extra-cellular infectious particles without affecting viral replication. Interestingly, the crude venom and its fractions is neither toxic nor hemolytic in vitro at a concentration 10,000-fold higher than that required for antiviral activity. Conclusively, this report highlights novel anti-HCV and anti-DENV activities of Naja haje venom and its derivatives for which may lay the foundation for developing a new therapeutic intervention against these flaviviruses.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
35
46
Evaluation of RAPD-Direct-PCR in Molecular Diagnosis of Bladder Cancer from Urinary Cell-Free DNA
Hamdy Aly , Hosny M. A. Behnsawy , Ahmed Moeen , Diaa A. Hameed , Mohsen A. Moustafa
For diseases of the urogenital tract, urine has been identified as an optimal liquid biopsy and noninvasive source of proteins, DNA and RNA. Nucleic acid isolation may lead to a reduced DNA yield or the presence of PCR inhibitors, leading to poor outcomes that might shift the integrity values of cell-free DNA (cf-DNA). Relying on KAPA Blood PCR Kit to directly bypassing DNA extraction, we utilized the Random Amplified Polymorphic DNA (RAPD) analysis, targeting the possible alterations of urinary cf-DNA in Bladder Cancer (BC) patients. Using an arbitrary primer of decamer OPC-01, the results showed unique profiles of amplified DNA fragments in urinary cf-DNA of BC patients compared to healthy controls. The RAPD fragments were detectable in 88% of the 26 patients while only in 50% of the 12 control samples. The largest detected fragment in control samples was 545-bp while the fragments of higher integrity up to 1525-bp were only detectable in patient samples. The results also showed 9 patient-specific bands that could serve as possible future targets for biomarker discovery of BC. By further analysis for validation and/or confirmation, some of these patient-specific fragments might find application in developing efficient RAPD primer/s for early diagnosis of BC.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
36
46
A simple PCR based Assay for Determination of Pre-implantation Embryonic Sex Using Cell-free DNA From Spent Culture Medium
Moustafa Sarhan , Mohammed Hesham , Hamdy Aly , Ali Gadel-Rab , Hesham Elshaer
molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
molecular biology research lab.,Zoology Dept., Faculty of Science, Al-Azhar University
Molecular biology research lab., Zoology Dept., Faculty of Science, Al-Azhar University
Zoology Dept., Faculty of Science, Al-Azhar University
Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University
ABSTRACT
Background: Pre-implantation genetic diagnosis (PGD) is a robust clinical tool to recognize embryos with risk of specific genetic diseases before implantation in utero after in vitro fertilization (IVF). PGD is usually performed on embryo biopsies of one or two cells obtained by aspiration from pre-implantation embryos at day 3 or day 5 of culture. However, non-invasive procedures to assess the genetic status of pre-implantation embryos are not available yet.
Aim of the work: We aimed to discriminate between female and male embryos by detecting Y chromosome-specific sequences in cell-free nucleic acids.
Materials and methods: Day 5 embryos were collected and cfDNA was extracted from each culture medium using QIAamp Circulating Nucleic Acid Kit. Embryonic sex was determined by PCR using SRY specific primers.
Results: CfDNA was successfully purified from all tested embryo culture samples and confirmed by PCR using human GAPDH specific primers. Using extracted cfDNA from embryonic culture medium and a simple PCR assay we were able to discriminate between male (XY) and female(XX) embryos.
Conclusion: This opens new doors for the development of a non-invasive method for prei-mplantation genetic screen.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
37
46
An investigation of potentially pathogenic Acanthamoeba species from a wastewater treatment plant in Alexandria, Egypt
Ahmad abdulaziz mohammad , Ahmad Zakaria Al-Herrawy , Mahmoud Afw Gad , Mohammed fathallah abou-elnour , Amgad salahuldeen abdulsayed
Zoology Department , Faculty of Science , Al-Azhar University,Cairo , Egypt.
Water Pollution Research Dept., National Research Centrer.
Water Pollution Research Dept., National Research Centrer.
Zoology Department , Faculty of Science , Al-Azhar University,Cairo , Egypt.
Zoology Department , Faculty of Science , Al-Azhar University,Cairo , Egypt.
ABSTRACT
From Wardian Wastewater Treatment Plant in Alexandria, 48 water samples were collected and filtered through nitrocellulose membranes, then, placed on non-nutrient agar with Escherichia coli for cultivation of FLAs. The obtained amoebae were morphologically identified and molecularly confirmed to the genus level. The results revealed that the occurrence of FLAs reached 75.0% and 62.5% in raw and treated wastewater samples, respectively. Morphological identification of FLAs from raw and treated wastewater samples showed that 45.8% and 37.5%, respectively (from the positive samples) belonging to genus Acanthamoeba. Whereas, the other FLAs in raw and treated water samples reached 29.2% and 25.0, respectively. The treatment water process of the plant removed only 12.5 % of FLAs. Statistically, seasons had no significant effect on the prevalence of FLAs in the wastewater samples. 85.0 % from the morphologically identified Acanthamoeba spp. were confirmed, when were tested using PCR. In conclusion, the presence of FLAs in treated wastewater leads to direct and indirect public health hazards as they may harbor other pathogenic microorganisms that can escape from the wastewater treatment processes and reach to the end user.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
38
46
SOME MITES ASSOCIATED WITH FOOD STUFF
Abdel-Sattar Mohammed Metwally, , Ahmed Saber Bream , Mohammed El-Sayed Gad.
Department of Agricultural Zoology and Nematology, Faculty of Agriculture, Al – Azhar University
Department of Zoology and Entomology, Faculty of science, Al – Azhar University.
ABSTRACT
This work was conducted to incidence the mites associated with stored food stuff during the two successive years (2018 and 2019). Twenty seven mite species from ten different stored products; wheat grain, wheat flour, wheat bran, rice grain, maize grain, pea, corn, bread bean, animal feed, and barley were collected from El-sharqia governorate. The collected mites belonging to one order Parasitiformes, one suborder Gamasida (Mesostigmata) and 7 families. Suborder Gamasida (Mesostigmata) included 27 species of 7 families.The collected mites belonging to fifteen genera. Order Parasitiformes including 7 families (Ascidae Vogits Oudemans, Ameroseiidae Evans, Lealapidae Berlese, Macrochelidae Vitzithum, Uropodidae Berlese, Rhodacaridae Oudemans and Parasitidae Oudemans).
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
39
46
The insecticidal activity of entomopathogenic bacteria isolated from Musca domestica, Chrysomya albiceps and Lucilia sericata against the mosquito larvae of Culex pipiens
Mohamed Ahmed Awad Bauni saad
Mohamed A. Awad
ABSTRACT
The present study evaluated the toxicity of different bacterial strains isolated from the outer surface of adult flies associated with carrions at Al – Mansoureya , Giza Governorate, Egypt against Culex pipiens larvae. 11 different bacterial strains were isolated and identified from the adults of Musca domestica, Chrysomya albiceps and Lucilia sericata. Only five strains caused mortality in C. pipiens larvae more than 50 % . The highest larval mortality was caused by the bacterial strains; Klebsiella oxytoca and Staphylococcus sciuri. The results indicated that entomopathogenic bacterial strains could have potential for the biological control of mosquitoes.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
40
46
BIOLOGICAL CONTROL OF LARVAL AND PUPAL STAGE OF PECTINOPHORA GOSSYPIELLA (SANDRES) (LEPIDOPTERA: GELECHIIDAE) WITH ENTOMOPATHOGENIC FUNGUS, BEAUVERIA BASSIANA
Moneir Saleh Amer , Ahmed Adly Ibrahim , Gamal Mohammed Omar
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
Plant Protection Research, Agriculture Research Center, Doki, Giza, Egypt
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
ABSTRACT
This experiment were carried out to study the effect of treatment newly hatched (neonate) larvae and pupal stage of the pink bollworm, Pectinophora gossypiella (Sandres) (Lepidoptera: Gelechiidae ) with different concentrations (108 , 109 , 1010 , 1011 and 1012) of entomopathogenic fungus Beauveria bassiana under laboratory conditions of 27±1 0C and 65±10 % R.H.
The results showed that, the entomopathogenic fungus Beauveria bassiana caused mortality percent of newly hatched (neonate) larvae 100% and 32.5 % with concentrations 1×1012 and 1×108 Spores/ml, respectively. The pupal mortality percent recorded were 85.0% and 35.0% when the pupal stage treated with concentration 1×1012 and 1×108 Spores/ml, respectively. This mortality percent increased as the concentration of the fungus increased.
These results suggest that Beauveria bassiana is highly effective in controlling populations of newly hatched (neonate) larvae and pupal stage of the pink bollworm, Pectinophora gossypiella.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
41
46
SOME ASTIGMATED PROSTIGMATED AND CRYPTOSTIGMATED MITES INHABITING SOME STORED PRODUCTS
Abdel-Sattar Mohammed Metwally , Ahmed Saber Bream , Mohammed El-Sayed Gad
Department of Agricultural Zoology and Nematology, Faculty of Agriculture, Al – Azhar University
Department of Zoology and Entomology, Faculty of science, Al – Azhar University
Department of Zoology and Entomology, Faculty of science, Al – Azhar University
ABSTRACT
This work was conducted to incidence the mites associated with stored food stuff during the two successive years (2018 and 2019). Thirty eight mite species from Twenty four different stored products; wheat grain, wheat flour, wheat bran, rice grain, maize grain, garlic, hay, onion, pea, cummin, corn, pea, bread bean, animal feed, poultry feed, carrot, egyption clover, straw, okra seeds, grass, watercress, potato, house dust and barley were collected from El-sharqia governorate. The collected mites belonging to one order Acariformes, 3 suborders and 15 families. Suborder Acaridida (Astigmata) included 21 species of 6 families; suborder Actinedida (prostigmata) included 12 species of 7 families; suborder Oribatida (Cryptostigmata) included 5 species of 2 families.
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
42
46
Therapeutic effect of Melissa Officinalis Against Hyperthyroidism Induced by Eltroxin In Male Albino Rats.
El-yamany I. El-zawahry , Khaled G. Abdel-Wahhab , Ahmed S. Hussein , Mohamed H.M. Abdel-Aziz
Zoology Department, Faculty of Science, (Boys) Al-Azhar University, Cairo, Egypt.
Medical Physiology Department, National Research Centre, Doki, Egypt.
Zoology Department, Faculty of Science, (Boys) Al-Azhar University, Cairo, Egypt.
Zoology Department, Faculty of Science, (Boys) Al-Azhar University, Cairo, Egypt.
ABSTRACT
Background: This study aimed to evaluate the protective effect of Melissa Officinalis Ethanolic Extract (MEE) in hyperthyroidism albino rats. Materials and Methods: After acclimatization, 50 male Wistar albino rats ranged from 150-170g were randomly divided into five groups; Group (1) normal control group (Control of healthy); Group (2) MEE administrated orally with (75 mg/kg) B.W; Group (3) hyperthyroidism-untreated group (positive control for hyperthyroidism), rats treated orally with Eltroxin® at (100 ug/kg) B.W; Group (4) hyperthyroidism treated group, rats were treated orally with tThyrocil® at the dose of 16.8 mg/kg/day dissolved in 1.0 ml saline and fed free and Group (5) hyperthyroidism rats model treated with MEE (75 mg/kg) dissolved in 1.0 ml saline and fed free. Results: Hyperthyroid group rats exhibited dramatic increase in thyroid-stimulating hormone (TSH) levels with concomitant reduction in the levels of thyroid hormones. However, treatment with the tThyrocil® and MEE respectively resulted in remarkable improvement in thyroid profile. According to lipid profile, oxidative stress and antioxidants levels as well as liver and kidney functions which obtained from hyperthyroidism-untreated group revealed that, there was a significant increase in the levels of serum cholesterol, LDL-C, alanine transaminase (ALT), aspartate transaminase (AST), urea, creatinine, uric acid, lipid peroxidation (MDA, NO, ATPase) in liver and kidney tissues whereas, a significant decrease in the levels of HDL-C, glutathione (GSH), glutathione peroxidase (GPX) liver and kidney tissues showed a significant decrease as compared to control group. On the other hand, the administration of MEE reduced serum total cholesterol, LDL-C, ALT, AST urea, creatinine, uric acid, lipid peroxidation (MDA, NO, ATPase) in liver and kidney tissues, moreover increased tissue GSH, GPX levels. Conclusions: These results establish that M. officinalis Ethanolic Extract (MEE) possesses potent antioxidative properties, validating its efficacy in attenuating oxidative stress as well as safe administration. Moreover, it has therapeutic potential to restore thyroid hormone levels and prevent the biochemical complications due to thyroid hormone insufficiency in the animal model of experimental hyperthyroidism.
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CSC
CHEM
GEO
BIO
ASM
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10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
43
46
Therapeutic role of bone marrow derived-mesenchymal stem cells and/or in combination with vitamin D in attenuation of pancreatic β-cell damage in diabetic rats induced by streptozotocin
Ahmed Saber Hussein , Elyamany Elzawahry , Mahmoud Salem , Sayed Bakry
Zoology Department, Faculty of Science, (Boys) Al-Azhar University, Cairo, Egypt.
Zoology Department, Faculty of Science, (Boys) Al-Azhar University, Cairo, Egypt.
Zoology Department, Faculty of Science, (Boys) Al-Azhar University, Cairo, Egypt.
Department of Medical Biochemistry, Faculty of Medicine, Cairo University, Kasr El Aini, Cairo, Egypt.
ABSTRACT
Background: Diabetes is one of the most important causes of mortality and morbidity all over the world. Renewal of functional pancreatic islets has been a goal of stem cell biologists since early 2000. Since that time, many studies have reported successful creation of glucose-responsive pancreatic beta-cells. Aim of work: This work aimed to study the effect of MSC.s alone and/or in combination with vitamin D3 in Streptozotocin (STZ)- induced diabetic male albino rats to detect its potential therapeutic effect and its possible application to humans. Material and methods: Twenty-four male albino rats (150 – 170 grams) were included in this study. They were divided into four equal groups; each group have six rats: Group I (Normal: control of healthy), group II (STZ: control of diabetes), group III (diabetic group post-treated with MSC.s) and group IV (diabetic group post-treated with MSC.s in combination with vitamin D3). Diabetes was induced by intraperitoneal injection of STZ (50 mg/kg); MSC.s were injected intravenously into the rat tail vein in group III and group IV then left for four weeks; vitamin D (cholecalciferol) was administered orally at 150 ng (500 IU/kg) each other day at three times per week during the experiment. Rats were sacrificed at week 4; lipid profile, blood glucose and insulin levels were estimated as well as histological examination of pancreatic sections of the experimental animals was analyzed for all groups at the end of the experiment. Results: Diabetic group (group II) showed significant higher glucose level as well as induce hyperlipidemia while there was a significant lower insulin level compared to control of non-diabetic group. Group III & group IV showed significant elevation of insulin level followed by reduction blood glucose level as well as amelioration of pancreatic β-cell architecture in compared to group II. Conclusion: treatment with MSC.s and/or in combination with vitamin D3 showed significant improvement of β- cell function and architecture in STZ induced hyperglycemia and hyperlipidemia as compared to diabetic untreated group.
Code
BOT
MATH
STA
CSC
CHEM
GEO
BIO
ASM
PHY
10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
44
46
“Protective effects of date palm extracts as natural antioxidants on hepatotoxicity induced by Cerastes cerastes venom in albino rats.”
Bashandy, M.A. , Abdel-Aal,A. , Ibrahim,D.F. , El-Sharkawy,M.A.
.Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
.Zoology Department, Faculty of Science, Ain-Shams University, Cairo, Egypt
.Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
.Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
ABSTRACT
The present study was designed to determine the possible protectiveeffects of date palm extract against Cerastes cerastes venom induced oxidative stress and biochemical changes in liver of albino rats. A patch of 104 male Wister albino rats averaged weights (130±10g) at the beginning of the experiment were divided into 6 main groups according to the treatmen and requirements of the experiment. Control rats group, DPE group treated orally with DPE at dose 1.2 mg/kg body weight, venoms groups: Rats injected with a single dose of Cerastes cerastes for one day, this group divided into three subgroups injected by a single dose of 1/4 LD50, 1/2 LD50 and LD50 for one day also for two weeks groups, venoms treated with DPE groups treated orally with DPE for two weeks before /and after injecting of a single dose of venom at dose 1/4 LD50, 1/2 LD50 and LD50 for one day. Each group contains 6 rats except venom groups of LD50 were 8 rats and six rats were sacrificed after the 1st day, 2nd and 4th week from each group of
treatment. The results showed a significant raise of some hepatic parameters (ALAT, ASAT, ALP, TG, CHO and LDL-C), while a significant reduction in some other parameters (Total protein, albumin and HDL-C), on the 1st day and 2nd weeks in rats injected intraperitoneal with Cerastes cerastes venom as compared to the control groups. The administration of the DPE ameliorated the side effects of the poisonous changes of Cerastes cerastes venom.
In conclusion, according to the results obtained revealed that the
administration of DPE had hepatoprotective effects against envenomation with Cerastes cerastes venom in male Wister albino rats by inhibiting oxidative stress through ROS scavenger and improvement of the biochemical markers
Code
BOT
MATH
STA
CSC
CHEM
GEO
BIO
ASM
PHY
10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
45
46
PARAPODIA SINAICA – TAMARIX NILOTICA INTERACTION AND MODELING OF THEIR HABITAT IN SOME REGIONS OF EGYPT
Ahmed S. Bream , Mohamed Kamel , Mohamed M. S. Morsy , Sanad H. Ragab
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
Department of Environmental Basic Science, Institute of Environmental Studies and Research, Ain Shams, Egypt
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr city, Cairo, Egypt
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
ABSTRACT
Galls and their inducing insects represent probably one of the most topics that facing entomologists because of their unique life history and the highly characteristic gall structure. Gall inducing insects and their distributions in Egypt of little studies which depend on few researchers from countries of Europe during their scientific journeys. Tamarix nilotica (family:Tamaricaceae) is known as Nile Tamarisk has diverse and medicinal uses in traditional medicine. Parapodia sinaica Frau. (Lepidoptera: Gelechiidae) is one of the most gall inducing insects that form spindle gall of Tamarix spp. The interaction between the gall inducer Parapodia sinaica and their host plant Tamarix nilotica were studied. The results demonstrate that there was significant correlation between number of galls per plant and plant cover but there was no correlation between number of galls per plant and altitude. The predicted distributions of Parapodia sinaica in Egypt was done by using MaxEnt technique. The results showed that the prediction distribution is concentrated in Delta Area.
Code
BOT
MATH
STA
CSC
CHEM
GEO
BIO
ASM
PHY
10th International Scientific Conf.
Basic Sciences and its Applications
30 March – 1 April, 2020
Cairo, Egypt
المؤتمر العلمي الدولي العاشر
العلوم الأساسية وتطبيقاتها
2020
ابريل
1
-
مارس
30
القاهرة ـ جمهورية مصر العربية
Zoology and its applications
46
46
STEFANIELLA TRINACRIAE – ATRIPLEX HALIMUS INTERACTION AND PREDICTING THE POTENTIAL GEOGRAPHIC DISTRIBUTION OF THE GALL INDUCING INSECT IN SOME REGIONS OF EGYPT BY USING MAXENT TECHNIQUE
Ahmed S. Bream , Mohamed Kamel , Mohamed M. Morsy , Sanad H. Ragab
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
Department of Environmental Basic Science, Institute of Environmental Studies and Research, Ain Shams, Egypt
Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr city, Cairo, Egypt
Zoology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt
ABSTRACT
In some regions of Mediterranean coast, Stefaniella trinacriae (Diptera: Cecidomyiidae) associated with one of the most important medicinal plants, the Atriplex halimus (Chenopodiaceae). S. trinacriae induces fusiform swellings galls on the stem of A. halimus with multiple chambers. In this paper relationship between the gall inducing insects Stefaniella trinacriae and their host plant A. halimus were studied. Our results demonstrated that elevation is avital variable determining the distribution of gall inducing insects S. trinacriae. The prediction of potential geographic distribution of gall inducing insects was built by using MaxEnt technique.
